TY - JOUR
T1 - One-end immobilization of individual dna molecules on a functional hydrophobic glass surface
AU - Matsuura, Shun Ichi
AU - Kurita, Hirofumi
AU - Nakano, Michihiko
AU - Komatsu, Jun
AU - Takashima, Kazunori
AU - Katsura, Shinji
AU - Mizuno, Akira
N1 - Funding Information:
This work was partially supported by a Grant-in-Aid for Scientific Research (No. 10638, 12450165, 14655307, and 13750390) from Japan Society for the Promotion of Science (JSPS) Research Fellowships and the Angstrom Technology Partnership of the Joint Research Center for Atom Technology (JRCAT). We are also grateful to Prof. K. Maeda of Kochi National College of Technology, Mr. H. Kumagai, Prof. Y. Husimi of Saitama University of Technology, Ms. A. Zdravkova, Prof. K. Nagayama of Okazaki National Research Institutes and Assoc. Prof. H. Tsuji of Toyohashi University of Technology for valuable suggestions and discussions.
PY - 2002/12
Y1 - 2002/12
N2 - We demonstrate an effective method for DNA immobilization on a hydrophobic glass surface. The new DNA immobilizing technique is extremely simple compared with conventional techniques that require heterobifunctional crosslinking reagent between DNA and substrate surface that are both modified chemically. In the first process, a coverslip was treated with dichlorodimethylsilane resulting in hydrophobic surface. γ DNA molecules were ligated with 3′-terminus disulfide-modified 14 mer oligonucleotides at one cohesive end. After reduction of the disulfide to sulfhydryl (thiol) groups the resulting thiol-modified γ DNA molecules were reacted on silanized coverslip. Fluorescent observation showed that the thiol-modified γ DNA molecules were anchored specifically to the hydrophobic surface at one terminus, although non-specific binding of the DNA molecules was suppressed. It was observed that the one-end-attached DNA molecule was bound firmly to the surface and stretched reversibly in one direction when a d.c. electric field was applied.
AB - We demonstrate an effective method for DNA immobilization on a hydrophobic glass surface. The new DNA immobilizing technique is extremely simple compared with conventional techniques that require heterobifunctional crosslinking reagent between DNA and substrate surface that are both modified chemically. In the first process, a coverslip was treated with dichlorodimethylsilane resulting in hydrophobic surface. γ DNA molecules were ligated with 3′-terminus disulfide-modified 14 mer oligonucleotides at one cohesive end. After reduction of the disulfide to sulfhydryl (thiol) groups the resulting thiol-modified γ DNA molecules were reacted on silanized coverslip. Fluorescent observation showed that the thiol-modified γ DNA molecules were anchored specifically to the hydrophobic surface at one terminus, although non-specific binding of the DNA molecules was suppressed. It was observed that the one-end-attached DNA molecule was bound firmly to the surface and stretched reversibly in one direction when a d.c. electric field was applied.
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U2 - 10.1080/07391102.2002.10506861
DO - 10.1080/07391102.2002.10506861
M3 - Article
C2 - 12437381
AN - SCOPUS:0036917593
VL - 20
SP - 429
EP - 436
JO - Journal of Biomolecular Structure and Dynamics
JF - Journal of Biomolecular Structure and Dynamics
SN - 0739-1102
IS - 3
ER -