Abstract
We demonstrate an effective method for DNA immobilization on a hydrophobic glass surface. The new DNA immobilizing technique is extremely simple compared with conventional techniques that require heterobifunctional crosslinking reagent between DNA and substrate surface that are both modified chemically. In the first process, a coverslip was treated with dichlorodimethylsilane resulting in hydrophobic surface. γ DNA molecules were ligated with 3′-terminus disulfide-modified 14 mer oligonucleotides at one cohesive end. After reduction of the disulfide to sulfhydryl (thiol) groups the resulting thiol-modified γ DNA molecules were reacted on silanized coverslip. Fluorescent observation showed that the thiol-modified γ DNA molecules were anchored specifically to the hydrophobic surface at one terminus, although non-specific binding of the DNA molecules was suppressed. It was observed that the one-end-attached DNA molecule was bound firmly to the surface and stretched reversibly in one direction when a d.c. electric field was applied.
| Original language | English |
|---|---|
| Pages (from-to) | 429-436 |
| Number of pages | 8 |
| Journal | Journal of Biomolecular Structure and Dynamics |
| Volume | 20 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 2002 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
Cite this
One-end immobilization of individual dna molecules on a functional hydrophobic glass surface. / Matsuura, Shun Ichi; Kurita, Hirofumi; Nakano, Michihiko; Komatsu, Jun; Takashima, Kazunori; Katsura, Shinji; Mizuno, Akira.
In: Journal of Biomolecular Structure and Dynamics, Vol. 20, No. 3, 01.01.2002, p. 429-436.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - One-end immobilization of individual dna molecules on a functional hydrophobic glass surface
AU - Matsuura, Shun Ichi
AU - Kurita, Hirofumi
AU - Nakano, Michihiko
AU - Komatsu, Jun
AU - Takashima, Kazunori
AU - Katsura, Shinji
AU - Mizuno, Akira
PY - 2002/1/1
Y1 - 2002/1/1
N2 - We demonstrate an effective method for DNA immobilization on a hydrophobic glass surface. The new DNA immobilizing technique is extremely simple compared with conventional techniques that require heterobifunctional crosslinking reagent between DNA and substrate surface that are both modified chemically. In the first process, a coverslip was treated with dichlorodimethylsilane resulting in hydrophobic surface. γ DNA molecules were ligated with 3′-terminus disulfide-modified 14 mer oligonucleotides at one cohesive end. After reduction of the disulfide to sulfhydryl (thiol) groups the resulting thiol-modified γ DNA molecules were reacted on silanized coverslip. Fluorescent observation showed that the thiol-modified γ DNA molecules were anchored specifically to the hydrophobic surface at one terminus, although non-specific binding of the DNA molecules was suppressed. It was observed that the one-end-attached DNA molecule was bound firmly to the surface and stretched reversibly in one direction when a d.c. electric field was applied.
AB - We demonstrate an effective method for DNA immobilization on a hydrophobic glass surface. The new DNA immobilizing technique is extremely simple compared with conventional techniques that require heterobifunctional crosslinking reagent between DNA and substrate surface that are both modified chemically. In the first process, a coverslip was treated with dichlorodimethylsilane resulting in hydrophobic surface. γ DNA molecules were ligated with 3′-terminus disulfide-modified 14 mer oligonucleotides at one cohesive end. After reduction of the disulfide to sulfhydryl (thiol) groups the resulting thiol-modified γ DNA molecules were reacted on silanized coverslip. Fluorescent observation showed that the thiol-modified γ DNA molecules were anchored specifically to the hydrophobic surface at one terminus, although non-specific binding of the DNA molecules was suppressed. It was observed that the one-end-attached DNA molecule was bound firmly to the surface and stretched reversibly in one direction when a d.c. electric field was applied.
UR - http://www.scopus.com/inward/record.url?scp=0036917593&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036917593&partnerID=8YFLogxK
U2 - 10.1080/07391102.2002.10506861
DO - 10.1080/07391102.2002.10506861
M3 - Article
C2 - 12437381
AN - SCOPUS:0036917593
VL - 20
SP - 429
EP - 436
JO - Journal of Biomolecular Structure and Dynamics
JF - Journal of Biomolecular Structure and Dynamics
SN - 0739-1102
IS - 3
ER -