One-end immobilization of individual dna molecules on a functional hydrophobic glass surface

Shun Ichi Matsuura, Hirofumi Kurita, Michihiko Nakano, Jun Komatsu, Kazunori Takashima, Shinji Katsura, Akira Mizuno

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We demonstrate an effective method for DNA immobilization on a hydrophobic glass surface. The new DNA immobilizing technique is extremely simple compared with conventional techniques that require heterobifunctional crosslinking reagent between DNA and substrate surface that are both modified chemically. In the first process, a coverslip was treated with dichlorodimethylsilane resulting in hydrophobic surface. γ DNA molecules were ligated with 3′-terminus disulfide-modified 14 mer oligonucleotides at one cohesive end. After reduction of the disulfide to sulfhydryl (thiol) groups the resulting thiol-modified γ DNA molecules were reacted on silanized coverslip. Fluorescent observation showed that the thiol-modified γ DNA molecules were anchored specifically to the hydrophobic surface at one terminus, although non-specific binding of the DNA molecules was suppressed. It was observed that the one-end-attached DNA molecule was bound firmly to the surface and stretched reversibly in one direction when a d.c. electric field was applied.

Original languageEnglish
Pages (from-to)429-436
Number of pages8
JournalJournal of Biomolecular Structure and Dynamics
Volume20
Issue number3
DOIs
Publication statusPublished - Jan 1 2002
Externally publishedYes

Fingerprint

Immobilization
Glass
DNA
Sulfhydryl Compounds
Disulfides
Cross-Linking Reagents
Oligonucleotides
Observation

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

One-end immobilization of individual dna molecules on a functional hydrophobic glass surface. / Matsuura, Shun Ichi; Kurita, Hirofumi; Nakano, Michihiko; Komatsu, Jun; Takashima, Kazunori; Katsura, Shinji; Mizuno, Akira.

In: Journal of Biomolecular Structure and Dynamics, Vol. 20, No. 3, 01.01.2002, p. 429-436.

Research output: Contribution to journalArticle

Matsuura, Shun Ichi ; Kurita, Hirofumi ; Nakano, Michihiko ; Komatsu, Jun ; Takashima, Kazunori ; Katsura, Shinji ; Mizuno, Akira. / One-end immobilization of individual dna molecules on a functional hydrophobic glass surface. In: Journal of Biomolecular Structure and Dynamics. 2002 ; Vol. 20, No. 3. pp. 429-436.
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