One-pot synthesis and characterization of laccase-entrapped magnetic nanobeads

Hideki Matsune; Hitoshi Jogasaki; Masato Date; Sakae Takenaka; Masahiro Kishida

doi:10.1246/cl.2006.1356

One-pot synthesis and characterization of laccase-entrapped magnetic nanobeads

Hideki Matsune, Hitoshi Jogasaki, Masato Date, Sakae Takenaka, Masahiro Kishida

Molecular and Biochemical Systems Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We report the simple one-pot synthesis of magnetic nanoparticles coated with silica-gel layers that entrap laccase (Lc) by the water-in-oil microemulsion method. The amorphous porous structure of the silica layer enables the entrapped Lc to react with substrates in solution. The enzymatic activity is studied using Michaelis-Menten kinetics. The reusability of the magnetic nanoparticles is examined.

Original language	English
Pages (from-to)	1356-1357
Number of pages	2
Journal	Chemistry Letters
Volume	35
Issue number	12
DOIs	https://doi.org/10.1246/cl.2006.1356
Publication status	Published - Dec 5 2006

All Science Journal Classification (ASJC) codes

Chemistry(all)

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10.1246/cl.2006.1356

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abstract = "We report the simple one-pot synthesis of magnetic nanoparticles coated with silica-gel layers that entrap laccase (Lc) by the water-in-oil microemulsion method. The amorphous porous structure of the silica layer enables the entrapped Lc to react with substrates in solution. The enzymatic activity is studied using Michaelis-Menten kinetics. The reusability of the magnetic nanoparticles is examined.",

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AU - Matsune, Hideki

AU - Jogasaki, Hitoshi

AU - Date, Masato

AU - Takenaka, Sakae

AU - Kishida, Masahiro

PY - 2006/12/5

Y1 - 2006/12/5

N2 - We report the simple one-pot synthesis of magnetic nanoparticles coated with silica-gel layers that entrap laccase (Lc) by the water-in-oil microemulsion method. The amorphous porous structure of the silica layer enables the entrapped Lc to react with substrates in solution. The enzymatic activity is studied using Michaelis-Menten kinetics. The reusability of the magnetic nanoparticles is examined.

AB - We report the simple one-pot synthesis of magnetic nanoparticles coated with silica-gel layers that entrap laccase (Lc) by the water-in-oil microemulsion method. The amorphous porous structure of the silica layer enables the entrapped Lc to react with substrates in solution. The enzymatic activity is studied using Michaelis-Menten kinetics. The reusability of the magnetic nanoparticles is examined.

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ER -

One-pot synthesis and characterization of laccase-entrapped magnetic nanobeads

Abstract

All Science Journal Classification (ASJC) codes

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