One-step synthesis of cellulose from cellobiose via protic acid-assisted enzymatic dehydration in aprotic organic media

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Direct and efficient enzymatic synthesis of long-chain cellulose from cellobiose in its original form was successfully achieved via the combination of a surfactant-enveloped enzyme (SEE) and a protic acid in an aprotic organic solvent, lithium chloride/N,N-dimethylacetamide system. The SEE biocatalyst was prepared by protecting the surface of cellulase with the nonionic surfactant dioleyl-N-d-glucona-l-glutamate for keeping its enzymatic activity in nonaqueous media. Fourier transform infrared and nuclear magnetic resonance analyses elucidated the successful synthesis of cellulose, β-1,4-linked d-glucopyranose polymer, through the reverse hydrolysis of cellobiose. By using protic acid cocatalysts, a degree of polymerization of as-synthesized cellulose reached more than 120, in a ca. 26% conversion, which was 5 times higher than that obtained in an acid-free SEE system. A novel-concept biocatalysis, i.e., a protic acid-assisted SEE-mediated reaction, enables a facile, one-step chain elongation of carbohydrates without any activation via multistep organic chemistry, and can provide potential applications in the functional design of glycomaterials.

Original languageEnglish
Pages (from-to)2716-2722
Number of pages7
JournalBiomacromolecules
Volume13
Issue number9
DOIs
Publication statusPublished - Sep 10 2012

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Cellobiose
Dehydration
Cellulose
Surface active agents
Enzymes
Surface-Active Agents
Acids
Biocatalysts
Nonionic surfactants
Lithium Chloride
Carbohydrates
Organic solvents
Cellulase
Elongation
Hydrolysis
Fourier transforms
Lithium
Chemical activation
Polymerization
Nuclear magnetic resonance

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

One-step synthesis of cellulose from cellobiose via protic acid-assisted enzymatic dehydration in aprotic organic media. / Egusa, Shizuka; Goto, Masahiro; Kitaoka, Takuya.

In: Biomacromolecules, Vol. 13, No. 9, 10.09.2012, p. 2716-2722.

Research output: Contribution to journalArticle

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