Opposite domination of cyclic and pseudocyclic electron flows in short-illuminated dark-adapted leaves of angiosperms and gymnosperms

Mari Noridomi, Shouta Nakamura, Michito Tsuyama, Norihiro Futamura, Radka Vladkova

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The present work was aimed to explain the recently reported higher O2-dependent electron flow capacity in gymnosperms than in angiosperms and to search for other differences in the electron transport processes by simultaneous characterization of the relative capacities of pseudocyclic (direct or Flavodiiron proteins (Flv)-mediated O2-reduction, Mehler(-like) reactions) and cyclic electron flows around photosystem I (CEF-PSI). To this end, a comparative multicomponent analysis was performed on the fluorescence decay curves of dark-adapted leaves after illumination with a 1-s saturating light pulse. In both gymnosperms and angiosperms, two or three exponential decay components were resolved: fast (t1/21 ~ 170–260 ms), middle (~1.0–2.3 s), and slow (>4.2 s). The sensitivity of the decay parameters (amplitudes A1–3, halftimes t1/2 1–3) to the alternative electron flows was assessed using Arabidopsis pgr5 and ndhM mutants, defective in CEF-PSI, Synechocystis sp. PCC 6803 Δflv1 mutant, defective in Flv-mediated O2-photoreduction, different O2 concentrations, and methyl viologen treatment. A1 reflected the part of electrons involved in linear and O2-photoreduction pathways after PSI. The middle component appeared in pgr5 (but not in ndhM), in gymnosperms under low O2, and in Δflv1, and reflected limitations at the PSI acceptor side. The slow component was sensitive to CEF-PSI. The comparison of decay parameters provided evidence that Flv mediate O2-photoreduction in gymnosperms, which explains their higher O2-dependent electron flow capacity. The concomitant quantification of relative electrons branching in O2-photoreduction and CEF-PSI pathways under the applied non-steady-state photosynthetic conditions reveals that CEF-PSI capacity significantly exceeds that of O2-photoreduction in angiosperms while the opposite occurs in gymnosperms.

Original languageEnglish
Pages (from-to)149-164
Number of pages16
JournalPhotosynthesis Research
Volume134
Issue number2
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

Gymnosperms
Angiosperms
Angiospermae
electrons
Electrons
Photosystem I Protein Complex
photosystem I
leaves
deterioration
Gymnospermae
Synechocystis sp. PCC 6803
Synechocystis
mutants
Paraquat
paraquat
Electron Transport
Lighting
Arabidopsis
electron transfer
lighting

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Opposite domination of cyclic and pseudocyclic electron flows in short-illuminated dark-adapted leaves of angiosperms and gymnosperms. / Noridomi, Mari; Nakamura, Shouta; Tsuyama, Michito; Futamura, Norihiro; Vladkova, Radka.

In: Photosynthesis Research, Vol. 134, No. 2, 01.11.2017, p. 149-164.

Research output: Contribution to journalArticle

Noridomi, Mari ; Nakamura, Shouta ; Tsuyama, Michito ; Futamura, Norihiro ; Vladkova, Radka. / Opposite domination of cyclic and pseudocyclic electron flows in short-illuminated dark-adapted leaves of angiosperms and gymnosperms. In: Photosynthesis Research. 2017 ; Vol. 134, No. 2. pp. 149-164.
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