Opsonic effect of the third complement component (C3) of carp (Cyprinus carpio) on phagocytosis by neutrophils

H. Matsuyama, T. Yano, T. Yamakawa, Miki Nakao

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Abstract

The opsonic effect of carp complement on the phagocytosis by carp peritoneal neutrophils was investigated using antibody-sensitised sheep erythrocytes (EA) and baker's yeast (Y), which are the activators of the classical complement pathway (CCP) and the alternative complement pathway (ACP), respectively. Carp antibody (A) exerted little opsonic effect on the phagocytosis of sheep erythrocytes (E). The treatment of EA and Y with normal carp serum (C) greatly enhanced phagocytosis. Such opsonic effect is apparently due to the complement being activated via CCP and ACP, because the treatment of EA and Y with C3-depleted carp serum produced no effect. It was also found that the treatment of EAC and YC with the Fab fragment of anti-carp C3 (rabbit IgG) abolished the opsonising effect of carp complement deposited on the target cells. These results indicate that C3 is the major phagocytosis-promoting factor (opsonin). In addition, it was also demonstrated that pretreatment of the neutrophil surface with trypsin greatly decreased the phagocytosis of EAC, suggesting the presence of C3 receptors on the neutrophil surface.

Original languageEnglish
Pages (from-to)69-78
Number of pages10
JournalFish and Shellfish Immunology
Volume2
Issue number1
DOIs
Publication statusPublished - Jan 1 1992

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phagocytosis
Cyprinus carpio
carp
neutrophils
complement
Opsonin Proteins
Immunoglobulin Fab Fragments
Antibodies
Yeast
Trypsin
Immunoglobulin G
sheep
antibody
serum
erythrocytes
opsonins
yeast
bakers yeast
antibodies
effect

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

Opsonic effect of the third complement component (C3) of carp (Cyprinus carpio) on phagocytosis by neutrophils. / Matsuyama, H.; Yano, T.; Yamakawa, T.; Nakao, Miki.

In: Fish and Shellfish Immunology, Vol. 2, No. 1, 01.01.1992, p. 69-78.

Research output: Contribution to journalArticle

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AB - The opsonic effect of carp complement on the phagocytosis by carp peritoneal neutrophils was investigated using antibody-sensitised sheep erythrocytes (EA) and baker's yeast (Y), which are the activators of the classical complement pathway (CCP) and the alternative complement pathway (ACP), respectively. Carp antibody (A) exerted little opsonic effect on the phagocytosis of sheep erythrocytes (E). The treatment of EA and Y with normal carp serum (C) greatly enhanced phagocytosis. Such opsonic effect is apparently due to the complement being activated via CCP and ACP, because the treatment of EA and Y with C3-depleted carp serum produced no effect. It was also found that the treatment of EAC and YC with the Fab fragment of anti-carp C3 (rabbit IgG) abolished the opsonising effect of carp complement deposited on the target cells. These results indicate that C3 is the major phagocytosis-promoting factor (opsonin). In addition, it was also demonstrated that pretreatment of the neutrophil surface with trypsin greatly decreased the phagocytosis of EAC, suggesting the presence of C3 receptors on the neutrophil surface.

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