Optical coherence tomography-based tissue dynamics imaging for longitudinal and drug response evaluation of tumor spheroids

IBrahim Abd El-Sadek, Arata Miyazawa, Larina Tzu Wei Shen, Shuichi Makita, Shinichi Fukuda, Toshiharu Yamashita, Yuki Oka, Pradipta Mukherjee, Satoshi Matsusaka, Tetsuro Oshika, Hideaki Kano, Yoshiaki Yasuno

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

We present optical coherence tomography (OCT)-based tissue dynamics imaging method to visualize and quantify tissue dynamics such as subcellular motion based on statistical analysis of rapid-time-sequence OCT signals at the same location. The analyses include logarithmic intensity variance (LIV) method and two types of OCT correlation decay speed analysis (OCDS). LIV is sensitive to the magnitude of the signal fluctuations, while OCDSs including early- and late-OCDS (OCDSe and OCDSl, respectively) are sensitive to the fast and slow tissue dynamics, respectively. These methods were able to visualize and quantify the longitudinal necrotic process of a human breast adenocarcinoma spheroid and its anti-cancer drug response. Additionally, the effects of the number of OCT signals and the total acquisition time on dynamics imaging are examined. Small number of OCT signals, e.g., five or nine suffice for dynamics imaging when the total acquisition time is suitably long.

Original languageEnglish
Pages (from-to)6231-6248
Number of pages18
JournalBiomedical Optics Express
Volume11
Issue number11
DOIs
Publication statusPublished - Nov 1 2020
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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