Optical microscopic observation of fluorescence enhanced by grating-coupled surface plasmon resonance

Keiko Tawa, Hironobu Hori, Kenji Kintaka, Kazuyuki Kiyosue, Yoshiro Tatsu, Junji Nishii

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

On the substrate carrying a sub-wavelength grating covered with a thin metal layer, a fluorescent dye-labeled cell was observed by fluorescence microscope. The fluorescence intensity was more than 20 times greater than that on an optically flat glass substrate. Such a great fluorescence enhancement from labeled cells bound to the grating substrate was due to the excitation by grating coupled surface plasmon resonance. The application of a grating substrate to two-dimensional detection and fluorescence microscopy appears to offer a promising method of taking highly sensitive fluorescence images.

Original languageEnglish
Pages (from-to)9781-9790
Number of pages10
JournalOptics Express
Volume16
Issue number13
DOIs
Publication statusPublished - Jun 23 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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