Optical microscopic observation of fluorescence enhanced by grating-coupled surface plasmon resonance

Keiko Tawa; Hironobu Hori; Kenji Kintaka; Kazuyuki Kiyosue; Yoshiro Tatsu; Junji Nishii

doi:10.1364/OE.16.009781

Optical microscopic observation of fluorescence enhanced by grating-coupled surface plasmon resonance

Keiko Tawa, Hironobu Hori, Kenji Kintaka, Kazuyuki Kiyosue, Yoshiro Tatsu, Junji Nishii

Research output: Contribution to journal › Article › peer-review

82 Citations (Scopus)

Abstract

On the substrate carrying a sub-wavelength grating covered with a thin metal layer, a fluorescent dye-labeled cell was observed by fluorescence microscope. The fluorescence intensity was more than 20 times greater than that on an optically flat glass substrate. Such a great fluorescence enhancement from labeled cells bound to the grating substrate was due to the excitation by grating coupled surface plasmon resonance. The application of a grating substrate to two-dimensional detection and fluorescence microscopy appears to offer a promising method of taking highly sensitive fluorescence images.

Original language	English
Pages (from-to)	9781-9790
Number of pages	10
Journal	Optics Express
Volume	16
Issue number	13
DOIs	https://doi.org/10.1364/OE.16.009781
Publication status	Published - Jun 23 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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10.1364/OE.16.009781

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abstract = "On the substrate carrying a sub-wavelength grating covered with a thin metal layer, a fluorescent dye-labeled cell was observed by fluorescence microscope. The fluorescence intensity was more than 20 times greater than that on an optically flat glass substrate. Such a great fluorescence enhancement from labeled cells bound to the grating substrate was due to the excitation by grating coupled surface plasmon resonance. The application of a grating substrate to two-dimensional detection and fluorescence microscopy appears to offer a promising method of taking highly sensitive fluorescence images.",

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AU - Tawa, Keiko

AU - Hori, Hironobu

AU - Kintaka, Kenji

AU - Kiyosue, Kazuyuki

AU - Tatsu, Yoshiro

AU - Nishii, Junji

PY - 2008/6/23

Y1 - 2008/6/23

N2 - On the substrate carrying a sub-wavelength grating covered with a thin metal layer, a fluorescent dye-labeled cell was observed by fluorescence microscope. The fluorescence intensity was more than 20 times greater than that on an optically flat glass substrate. Such a great fluorescence enhancement from labeled cells bound to the grating substrate was due to the excitation by grating coupled surface plasmon resonance. The application of a grating substrate to two-dimensional detection and fluorescence microscopy appears to offer a promising method of taking highly sensitive fluorescence images.

AB - On the substrate carrying a sub-wavelength grating covered with a thin metal layer, a fluorescent dye-labeled cell was observed by fluorescence microscope. The fluorescence intensity was more than 20 times greater than that on an optically flat glass substrate. Such a great fluorescence enhancement from labeled cells bound to the grating substrate was due to the excitation by grating coupled surface plasmon resonance. The application of a grating substrate to two-dimensional detection and fluorescence microscopy appears to offer a promising method of taking highly sensitive fluorescence images.

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SN - 1094-4087

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Optical microscopic observation of fluorescence enhanced by grating-coupled surface plasmon resonance

Abstract

All Science Journal Classification (ASJC) codes

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