Optical resolution of various amino acids using a supported liquid membrane encapsulating a surfactant-protease complex

Eijiro Miyako, Tatsuo Maruyama, Fukiko Kubota, Noriho Kamiya, Masahiro Goto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have encapsulated a surfactant-protease complex (the main protease used being a-chymotrypsin) in an organic phase of a supported liquid membrane (SLM) for the optical resolution of various amino acids. L-Isomers of amino acids were enantioselectively permeated through the SLM. The mechanism of the amino acid permeation through the SLM was considered to be as follows; an L-amino acid was enantioselectively esterified with ethanol by a surfactant-protease complex encapsulated in the SLM, and the resulting L-amino acid ethyl ester dissolved into the organic phase of the SLM and diffused across the SLM. Another surfactant-α-chymotrypsin complex in the receiving phase catalyzed ester hydrolysis to produce the initial L-amino acid and ethanol, which are water-soluble. Thus, the L-amino acid was selectively transported to the receiving phase through the SLM on the basis of the molecular recognition of the surfactant-protease complex in the SLM. It was found that the catalytic activity and enantioselectivity of the surfactant-protease complex governed the permeate flux of amino acids and the enantiomeric excess in the membrane separation.

Original languageEnglish
Pages (from-to)4674-4679
Number of pages6
JournalLangmuir
Volume21
Issue number10
DOIs
Publication statusPublished - May 10 2005

Fingerprint

protease
Liquid membranes
encapsulating
Surface-Active Agents
amino acids
Amino acids
Surface active agents
Peptide Hydrolases
surfactants
membranes
Amino Acids
liquids
Chymotrypsin
Esters
Ethanol
esters
ethyl alcohol
Molecular recognition
Enantioselectivity
Permeation

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Optical resolution of various amino acids using a supported liquid membrane encapsulating a surfactant-protease complex. / Miyako, Eijiro; Maruyama, Tatsuo; Kubota, Fukiko; Kamiya, Noriho; Goto, Masahiro.

In: Langmuir, Vol. 21, No. 10, 10.05.2005, p. 4674-4679.

Research output: Contribution to journalArticle

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