Optical tweezers with fluorescence detection for temperature-dependent microrheological measurements

Atsuomi Shundo, Koichiro Hori, David P. Penaloza, Keiji Tanaka

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We introduce a setup of optical tweezers, capable of carrying out temperature-dependent rheological measurements of soft materials. In our setup, the particle displacement is detected by imaging a bright spot due to fluorescence emitted from a dye-labeled particle against a dark background onto a quadrant photodiode. This setup has a relatively wide space around the sample that allows us to further accessorize the optical tweezers by a temperature control unit. The applicability of the setup was examined on the basis of the rheological measurements using a typical viscoelastic system, namely a worm-like micelle solution. The temperature and frequency dependences of the local viscoelastic functions of the worm-like micelle solution obtained by this setup were in good accordance with those obtained by a conventional oscillatory rheometer, confirming the capability of the optical tweezers as a tool for the local rheological measurements of soft materials. Since the optical tweezers measurements only require a tiny amount of sample (∼40 μL), the rheological measurements using our setup should be useful for soft materials of which the available amount is limited.

Original languageEnglish
Article number014103
JournalReview of Scientific Instruments
Volume84
Issue number1
DOIs
Publication statusPublished - Jan 1 2013

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Optical tweezers
Fluorescence
fluorescence
worms
Micelles
Temperature
temperature
micelles
Rheometers
Photodiodes
rheometers
Temperature control
quadrants
temperature control
optical measurement
Dyes
photodiodes
Imaging techniques
dyes
temperature dependence

All Science Journal Classification (ASJC) codes

  • Instrumentation

Cite this

Optical tweezers with fluorescence detection for temperature-dependent microrheological measurements. / Shundo, Atsuomi; Hori, Koichiro; Penaloza, David P.; Tanaka, Keiji.

In: Review of Scientific Instruments, Vol. 84, No. 1, 014103, 01.01.2013.

Research output: Contribution to journalArticle

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