Abstract
We investigated optimization of the feeding of L-lactic acid for the production of poly-D-3-hydroxybutyric acid [P(3HB)] by Alcaligenes eutrophus in a fed-batch culture system. An acidic substrate solution was fed automatically so as to maintain the pH of the culture liquid at 7.0. Feeding of a substrate solution containing 45% (w/v) L-lactic acid, 6.2% (w/v) sodium L-lactate, 5.8% (w/v) ammonia water and 1.8% (w/v) potassium phosphate [at a molar ratio of carbon to nitrogen (C/N molar ratio) of 10], allowed the L-lactate concentration in the culture liquid to be maintained at approximately 2 g/l and the cell concentration reached 27.4 g/l after 15 h of cultivation. To promote P(3HB) production, a two-stage fed-batch culture consisting of a culture for cell growth and one for P(3HB) accumulation was carried out. When the substrate solution, whose C/N molar ratio was 23, was fed during the P(3HB) accumulation phase, the cell concentration and the P(3HB) content in the cells reached 103 g/l and 57.6% (w/w), respectively, in 51.5 h.
| Original language | English |
|---|---|
| Pages (from-to) | 404-409 |
| Number of pages | 6 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 88 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jan 1 1999 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Optimization of L-lactic acid feeding for the production of poly-D-3- hydroxybutyric acid by Alcaligenes eutrophus in fed-batch culture. / Tsuge, Takeharu; Tanaka, Kenji; Shimoda, Mitsuya; Ishizaki, Ayaaki.
In: Journal of Bioscience and Bioengineering, Vol. 88, No. 4, 01.01.1999, p. 404-409.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optimization of L-lactic acid feeding for the production of poly-D-3- hydroxybutyric acid by Alcaligenes eutrophus in fed-batch culture
AU - Tsuge, Takeharu
AU - Tanaka, Kenji
AU - Shimoda, Mitsuya
AU - Ishizaki, Ayaaki
PY - 1999/1/1
Y1 - 1999/1/1
N2 - We investigated optimization of the feeding of L-lactic acid for the production of poly-D-3-hydroxybutyric acid [P(3HB)] by Alcaligenes eutrophus in a fed-batch culture system. An acidic substrate solution was fed automatically so as to maintain the pH of the culture liquid at 7.0. Feeding of a substrate solution containing 45% (w/v) L-lactic acid, 6.2% (w/v) sodium L-lactate, 5.8% (w/v) ammonia water and 1.8% (w/v) potassium phosphate [at a molar ratio of carbon to nitrogen (C/N molar ratio) of 10], allowed the L-lactate concentration in the culture liquid to be maintained at approximately 2 g/l and the cell concentration reached 27.4 g/l after 15 h of cultivation. To promote P(3HB) production, a two-stage fed-batch culture consisting of a culture for cell growth and one for P(3HB) accumulation was carried out. When the substrate solution, whose C/N molar ratio was 23, was fed during the P(3HB) accumulation phase, the cell concentration and the P(3HB) content in the cells reached 103 g/l and 57.6% (w/w), respectively, in 51.5 h.
AB - We investigated optimization of the feeding of L-lactic acid for the production of poly-D-3-hydroxybutyric acid [P(3HB)] by Alcaligenes eutrophus in a fed-batch culture system. An acidic substrate solution was fed automatically so as to maintain the pH of the culture liquid at 7.0. Feeding of a substrate solution containing 45% (w/v) L-lactic acid, 6.2% (w/v) sodium L-lactate, 5.8% (w/v) ammonia water and 1.8% (w/v) potassium phosphate [at a molar ratio of carbon to nitrogen (C/N molar ratio) of 10], allowed the L-lactate concentration in the culture liquid to be maintained at approximately 2 g/l and the cell concentration reached 27.4 g/l after 15 h of cultivation. To promote P(3HB) production, a two-stage fed-batch culture consisting of a culture for cell growth and one for P(3HB) accumulation was carried out. When the substrate solution, whose C/N molar ratio was 23, was fed during the P(3HB) accumulation phase, the cell concentration and the P(3HB) content in the cells reached 103 g/l and 57.6% (w/w), respectively, in 51.5 h.
UR - http://www.scopus.com/inward/record.url?scp=0032746261&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032746261&partnerID=8YFLogxK
U2 - 10.1016/S1389-1723(99)80217-5
DO - 10.1016/S1389-1723(99)80217-5
M3 - Article
C2 - 16232635
AN - SCOPUS:0032746261
VL - 88
SP - 404
EP - 409
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 4
ER -