Optimization of medium for the production of a novel aquaculture probiotic, streptomyces sp. a1 using central composite design of response surface methodology

Ngo Thi Tuong Chau, Masaru Matsumoto, Ikuo Miyajima

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A strain, Streptomyces sp. A1 isolated from shrimp pond sediments has been identified as an aquaculture probiotic antagonistic to pathogenic Vibrio harveyi V7. In the present study, the different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity, following response surface methodology (RSM). Accordingly, the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design CCD of RSM for the final optimization. The response surface plots of biomass production showed similar pattern with that of antagonistic activity, which indicated a correlation between the biomass and antagonism. The optimum concentrations of the carbon and nitrogen source, and growth factors for both biomass and antagonistic activity were starch (14.62 g/1), casein (0.93 g/1), NaCl (15.6 g/1), DL-α-alanine (0.233 g/1), and vitamin B6 (0.023 g/1).

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalJournal of the Faculty of Agriculture, Kyushu University
Issue number1
Publication statusPublished - Feb 1 2014


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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