Optimization of the inter-domain structure of galectin-9 for recombinant production

Aiko Itoh, Yoko Fukata, Hiroshi Miyanaka, Yasuhiro Nonaka, Takashi Ogawa, Takanori Nakamura, Nozomu Nishi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We previously developed a stable form of galectin-9, an immunomodulatory animal lectin with a truncated linker peptide (G9Null), to overcome the protease sensitivity of wild-type galectin-9. G9Null is highly resistant to proteolysis, while the modification marginally improved the low solubility of the wild-type protein. To increase its solubility, we further modified the remaining linker region of G9Null. A 10-amino acid deletion with a single amino acid substitution resulted in an ∼400% increase in solubility and yield without an adverse effect on its biological activity. This mutant protein might be useful for large-scale recombinant production needed for evaluation of the therapeutic potential of galectin-9.

Original languageEnglish
Pages (from-to)920-925
Number of pages6
JournalGlycobiology
Volume23
Issue number8
DOIs
Publication statusPublished - Aug 1 2013

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Galectins
Solubility
Proteolysis
Amino Acids
Mutant Proteins
Amino Acid Substitution
Bioactivity
Lectins
Peptide Hydrolases
Substitution reactions
Peptides
Proteins
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Itoh, A., Fukata, Y., Miyanaka, H., Nonaka, Y., Ogawa, T., Nakamura, T., & Nishi, N. (2013). Optimization of the inter-domain structure of galectin-9 for recombinant production. Glycobiology, 23(8), 920-925. https://doi.org/10.1093/glycob/cwt023

Optimization of the inter-domain structure of galectin-9 for recombinant production. / Itoh, Aiko; Fukata, Yoko; Miyanaka, Hiroshi; Nonaka, Yasuhiro; Ogawa, Takashi; Nakamura, Takanori; Nishi, Nozomu.

In: Glycobiology, Vol. 23, No. 8, 01.08.2013, p. 920-925.

Research output: Contribution to journalArticle

Itoh, A, Fukata, Y, Miyanaka, H, Nonaka, Y, Ogawa, T, Nakamura, T & Nishi, N 2013, 'Optimization of the inter-domain structure of galectin-9 for recombinant production', Glycobiology, vol. 23, no. 8, pp. 920-925. https://doi.org/10.1093/glycob/cwt023
Itoh A, Fukata Y, Miyanaka H, Nonaka Y, Ogawa T, Nakamura T et al. Optimization of the inter-domain structure of galectin-9 for recombinant production. Glycobiology. 2013 Aug 1;23(8):920-925. https://doi.org/10.1093/glycob/cwt023
Itoh, Aiko ; Fukata, Yoko ; Miyanaka, Hiroshi ; Nonaka, Yasuhiro ; Ogawa, Takashi ; Nakamura, Takanori ; Nishi, Nozomu. / Optimization of the inter-domain structure of galectin-9 for recombinant production. In: Glycobiology. 2013 ; Vol. 23, No. 8. pp. 920-925.
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