Optimum design of amphiphilic polymers bearing hydrophobic groups for both cell surface ligand presentation and intercellular cross-linking

Masafumi Takeo, Cuicui Li, Masayoshi Matsuda, Hiroko Nagai, Wataru Hatanaka, Tatsuhiro Yamamoto, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Amphiphilic polymers bearing hydrophobic alkyl groups are expected to be applicable for both ligand presentation on the cell surface and intercellular crosslinking. To explore the optimum design for each application, we synthesized eight different acyl-modified dextrans with varying molecular weight, alkyl length, and alkyl modification degree. We found that the behenate-modified polymers retained on the cell surface longer than the palmitate-modified ones. Since the polymers were also modified with biotin, streptavidin can be presented on the cell surface through biotin-streptavidin recognition. The duration of streptavidin on the cell surface is longer in the behenate-modified polymer than the palmitate-modified one. As for the intercellular crosslinking, the palmitate-modified polymers were more efficient than the behenate-modified polymers. The findings in this research will be helpful to design the acyl-modified polymers for the cell surface engineering.

Original languageEnglish
Pages (from-to)353-368
Number of pages16
JournalJournal of Biomaterials Science, Polymer Edition
Volume26
Issue number6
DOIs
Publication statusPublished - Apr 13 2015

Fingerprint

Bearings (structural)
Polymers
Ligands
Streptavidin
Palmitates
Biotin
Crosslinking
Cell Engineering
Dextran
Optimum design
Dextrans
Molecular Weight
Molecular weight

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Optimum design of amphiphilic polymers bearing hydrophobic groups for both cell surface ligand presentation and intercellular cross-linking. / Takeo, Masafumi; Li, Cuicui; Matsuda, Masayoshi; Nagai, Hiroko; Hatanaka, Wataru; Yamamoto, Tatsuhiro; Kishimura, Akihiro; Mori, Takeshi; Katayama, Yoshiki.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 26, No. 6, 13.04.2015, p. 353-368.

Research output: Contribution to journalArticle

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AU - Hatanaka, Wataru

AU - Yamamoto, Tatsuhiro

AU - Kishimura, Akihiro

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