Oral administration of bovine milk from cows hyperimmunized with intestinal bacterin stimulates lamina propria T lymphocytes to produce Th1-biased cytokines in mice

Yuanyuan Wang, Lianjie Lin, Chunming Yin, Satoru Othtani, Katsuhiko Aoyama, Changlong Lu, Xun Sun, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    The goal of this study was to examine the effects of oral administration of bovine milk from cows hyperimmunized with a proprietary bacterin (immune milk "Sustaina") on mucosal immunity in the intestine of adult mice. C57BL/6 mice were orally given immune or control milk for two weeks, and then lymphocyte population and the cytokine production in lamina propria of colon in normal mice and mice induced colitis by dextran sulphate sodium (DSS) were detected. We found that the levels of IFN-γ and IL-10 increased, but the levels of IL-17A and IL-4, decreased in lamina propria of colon in immune milk-fed mice as compared with those in control milk-fed mice. Interestingly, oral administration of immune milk partially improved the acute colitis induced by DSS. The levels of TNF-α and IFN-γ increased, but IL-6, IL-17A and IL-4 decreased in lamina propria (LP) of colon in immune milk-fed mice with DSS-induced colitis. Our results suggest that immune milk may stimulate CD4+ T cells to polarize towards a Th1 type response, but contrarily suppress Th17 and Th2 cells responses in large intestinal LP of mice. The results indicate that this kind of immune milk has is able to promote the maintainance of intestinal homeostasis and enhance protection against infection, and could alleviate the symptoms of acute colitis in mice.

    Original languageEnglish
    Pages (from-to)5458-5471
    Number of pages14
    JournalInternational journal of molecular sciences
    Volume15
    Issue number4
    DOIs
    Publication statusPublished - Mar 28 2014

    Fingerprint

    Bacterial Vaccines
    milk
    T-cells
    lymphocytes
    Oral Administration
    mice
    Milk
    Mucous Membrane
    Cytokines
    T-Lymphocytes
    Colitis
    Dextran Sulfate
    dextrans
    Sodium sulfate
    Dextran
    sodium sulfates
    Colon
    Interleukin-17
    Interleukin-4
    Th17 Cells

    All Science Journal Classification (ASJC) codes

    • Catalysis
    • Molecular Biology
    • Spectroscopy
    • Computer Science Applications
    • Physical and Theoretical Chemistry
    • Organic Chemistry
    • Inorganic Chemistry

    Cite this

    Oral administration of bovine milk from cows hyperimmunized with intestinal bacterin stimulates lamina propria T lymphocytes to produce Th1-biased cytokines in mice. / Wang, Yuanyuan; Lin, Lianjie; Yin, Chunming; Othtani, Satoru; Aoyama, Katsuhiko; Lu, Changlong; Sun, Xun; Yoshikai, Yasunobu.

    In: International journal of molecular sciences, Vol. 15, No. 4, 28.03.2014, p. 5458-5471.

    Research output: Contribution to journalArticle

    Wang, Yuanyuan ; Lin, Lianjie ; Yin, Chunming ; Othtani, Satoru ; Aoyama, Katsuhiko ; Lu, Changlong ; Sun, Xun ; Yoshikai, Yasunobu. / Oral administration of bovine milk from cows hyperimmunized with intestinal bacterin stimulates lamina propria T lymphocytes to produce Th1-biased cytokines in mice. In: International journal of molecular sciences. 2014 ; Vol. 15, No. 4. pp. 5458-5471.
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