Oral administration of xanthan gum enhances antitumor activity through Toll-like receptor 4

ario takeuchi, Yoriyuki Kamiryou, Hisakata Yamada, Masatoshi Eto, Kensuke Shibata, Kennichi Haruna, Seiji Naito, Yasunobu Yoshikai

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Abstract

Purpose: Xanthan gum (XG) is a complex exopolysaccharide produced by the plant-pathogenic bacterium Xanthomonas campestris pv. and is widely used as a thickener or viscosifier. We examined in this study the antitumor effects of XG. Experimental design: Cytokine production by XG-stimulated murine macrophage cell lines, J772 and RAW264.7, and peritoneal adherent cells from wild type C57BL/6 mice, TLR2 or MyD88-deficient mice, C3H/HeN, and TLR4-mutant C3H/HeJ mice were examined. In order to examine in vivo antitumor effects of XG, mice were inoculated subcutaneously with tumor cells and administered orally with XG once every 5 days from 1 day before the tumor inoculation. Tumor growth, mouse survival, NK activity, and tumor-specific cytotoxicity were examined. Results: In vitro culture with XG induced interleukin-12 and tumor necrosis factor-alpha production from macrophages. XG stimulated macrophages in a MyD88-dependent manner and was mainly recognized by Toll-like receptor 4 (TLR4). Oral administration of XG significantly retarded tumor growth and prolonged survival of the mice inoculated subcutaneously with B16Kb melanoma cells. NK activity as well as tumor-specific cytotoxicity of CD8 T cells was augmented in the XG-treated mice. The in vivo antitumor effects of XG were also dependent on TLR-4, as C3H/HeJ mice, which lack TLR4 signaling, exhibited no effect of XG on the growth of syngeneic bladder tumor, MBT-2. Conclusion: These results suggest beneficial effects of oral administration of XG on immune-surveillance against neoplasms.

Original languageEnglish
Pages (from-to)1562-1567
Number of pages6
JournalInternational Immunopharmacology
Volume9
Issue number13-14
DOIs
Publication statusPublished - Dec 1 2009

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Toll-Like Receptor 4
Oral Administration
Inbred C3H Mouse
Neoplasms
Macrophages
xanthan gum
Growth
Xanthomonas campestris
Interleukin-12
Inbred C57BL Mouse
Urinary Bladder Neoplasms
Melanoma
Research Design
Tumor Necrosis Factor-alpha

All Science Journal Classification (ASJC) codes

  • Immunology
  • Immunology and Allergy
  • Pharmacology

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Oral administration of xanthan gum enhances antitumor activity through Toll-like receptor 4. / takeuchi, ario; Kamiryou, Yoriyuki; Yamada, Hisakata; Eto, Masatoshi; Shibata, Kensuke; Haruna, Kennichi; Naito, Seiji; Yoshikai, Yasunobu.

In: International Immunopharmacology, Vol. 9, No. 13-14, 01.12.2009, p. 1562-1567.

Research output: Contribution to journalArticle

takeuchi, ario ; Kamiryou, Yoriyuki ; Yamada, Hisakata ; Eto, Masatoshi ; Shibata, Kensuke ; Haruna, Kennichi ; Naito, Seiji ; Yoshikai, Yasunobu. / Oral administration of xanthan gum enhances antitumor activity through Toll-like receptor 4. In: International Immunopharmacology. 2009 ; Vol. 9, No. 13-14. pp. 1562-1567.
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