Oral CD103 - CD11b + classical dendritic cells present sublingual antigen and induce Foxp3 + regulatory T cells in draining lymph nodes

Y. Tanaka, H. Nagashima, K. Bando, L. Lu, A. Ozaki, Y. Morita, Satoshi Fukumoto, N. Ishii, S. Sugawara

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Sublingual immunotherapy (SLIT) is a safe and efficient treatment for type 1 allergies; however, the underlying immunological mechanisms, particularly the phenotype of oral antigen-presenting cells (APCs) responsible for the induction of regulatory T (Treg) cells, remain unclear. We show here that the sublingual application of ovalbumin (OVA) induced antigen-specific Foxp3 + Treg cells in draining submandibular lymph nodes (ManLNs). Oral APCs were classified into macrophages, classical dendritic cells (cDCs), and Langerhans cells by flow cytometry. A major subset of oral cDCs with the CD103 - CD11b + phenotype showed retinoic acid (RA)-producing activity and converted naive CD4 + T cells to Foxp3 + Treg cells in a transforming growth factor-β- and RA-dependent manner in vitro. In the ManLNs, migratory CD103 - CD11b + cDCs also showed RA-producing activity. After the sublingual application of fluorescent OVA, fluorescence was detected in oral macrophages in tissues, followed by migratory CD103 - CD11b + cDCs in ManLNs and migratory CD103 - CD11b + cDCs were the main APCs responsible for the induction of sublingual antigen-specific Treg cells. The transfer of OVA-SLIT-induced Treg cells suppressed the OVA-induced hypersensitivity response. These results suggest that oral CD103 - CD11b + cDCs transport sublingual antigens to draining ManLNs and induce antigen-specific Foxp3 + Treg cells, and, thus, provide a rationale for developing cDC-based therapeutic approaches in SLIT.

Original languageEnglish
Pages (from-to)79-90
Number of pages12
JournalMucosal Immunology
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1 2017
Externally publishedYes

Fingerprint

Regulatory T-Lymphocytes
Dendritic Cells
Lymph Nodes
Antigens
Ovalbumin
Sublingual Immunotherapy
Antigen-Presenting Cells
Tretinoin
Hypersensitivity
Macrophages
Phenotype
Langerhans Cells
Transforming Growth Factors
Flow Cytometry
Fluorescence
T-Lymphocytes
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Oral CD103 - CD11b + classical dendritic cells present sublingual antigen and induce Foxp3 + regulatory T cells in draining lymph nodes. / Tanaka, Y.; Nagashima, H.; Bando, K.; Lu, L.; Ozaki, A.; Morita, Y.; Fukumoto, Satoshi; Ishii, N.; Sugawara, S.

In: Mucosal Immunology, Vol. 10, No. 1, 01.01.2017, p. 79-90.

Research output: Contribution to journalArticle

Tanaka, Y. ; Nagashima, H. ; Bando, K. ; Lu, L. ; Ozaki, A. ; Morita, Y. ; Fukumoto, Satoshi ; Ishii, N. ; Sugawara, S. / Oral CD103 - CD11b + classical dendritic cells present sublingual antigen and induce Foxp3 + regulatory T cells in draining lymph nodes. In: Mucosal Immunology. 2017 ; Vol. 10, No. 1. pp. 79-90.
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