Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle

Reiko Nakao, Weilin Shen, Yasuka Shimajiri, Kumiko Kainou, Yuki Sato, Anayt Ulla, Kohta Ohnishi, Miyuki Ninomiya, Ayako Ohno, Takayuki Uchida, Mitsuru Tanaka, Kazuhito Akama, Toshiro Matsui, Takeshi Nikawa

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We previously reported that intramuscular injections of ubiquitin ligase CBLB inhibitory pentapeptide (Cblin; Asp-Gly-pTyr-Met-Pro) restored lost muscle mass caused by sciatic denervation. Here, we detected Cblin on the basolateral side of Caco-2 cells after being placed on the apical side, and found that cytochalasin D, a tight junction opener, enhanced Cblin transport. Orally administered Cblin was found in rat plasma, indicating that intact Cblin was absorbed in vitro and in vivo. Furthermore, transgenic Cblin peptide-enriched rice (CbR) prevented the denervation-induced loss of muscle mass and the upregulation of muscle atrophy-related ubiquitin ligases in mice. These findings indicated that CbR could serve as an alternative treatment for muscle atrophy.

Original languageEnglish
Article number25
Journalnpj Science of Food
Volume5
Issue number1
DOIs
Publication statusPublished - Dec 2021

All Science Journal Classification (ASJC) codes

  • Food Science
  • Public Health, Environmental and Occupational Health

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