Oral lipase activities and fat-taste receptors for fat-taste sensing in chickens

Yuko Kawabata, Fuminori Kawabata, Shotaro Nishimura, Shoji Tabata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

It has been reported that a functional fat-taste receptor, GPR120, is present in chicken oral tissues, and that chickens can detect fat taste in a behavioral test. However, although triglycerides need to be digested to free fatty acids to be recognized by fat-taste receptors such as GPR120, it remains unknown whether lipase activities exist in chicken oral tissues. To examine this question, we first cloned another fat-taste receptor candidate gene, CD36, from the chicken palate. Then, using RT-PCR, we determined that GPR120 and CD36 were broadly expressed in chicken oral and gastrointestinal tissues. Also by RT-PCR, we confirmed that several lipase genes were expressed in both oral and gastrointestinal tissues. Finally, we analyzed the lipase activities of oral tissues by using a fluorogenic triglyceride analog as a lipase substrate. We found there are functional lipases in oral tissues as well as in the stomach and pancreas. These results suggested that chickens have a basic fat-taste reception system that incorporates a triglycerides/oral-lipases/free fatty acids/GPR120 axis and CD36 axis.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume495
Issue number1
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Chickens
Lipase
Fats
Tissue
Triglycerides
Nonesterified Fatty Acids
Genes
Polymerase Chain Reaction
Palate
pregastric esterase
Pancreas
Stomach
Substrates

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Oral lipase activities and fat-taste receptors for fat-taste sensing in chickens. / Kawabata, Yuko; Kawabata, Fuminori; Nishimura, Shotaro; Tabata, Shoji.

In: Biochemical and Biophysical Research Communications, Vol. 495, No. 1, 01.01.2018, p. 131-135.

Research output: Contribution to journalArticle

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