Orally administered l-ornithine reduces restraint stress-induced activation of the hypothalamic-pituitary-adrenal axis in mice

Koji Kurata, Mao Nagasawa, Shozo Tomonaga, Mami Aoki, Saori Akiduki, Koji Morishita, D. Michael Denbow, Mitsuhiro Furuse

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10 Citations (Scopus)

Abstract

In a previous study, we confirmed that orally administered l-ornithine can be transported into the brain of mice. In addition, orally administered l-ornithine, within a limited dose range, had an anxiolytic-like effect in the elevated plus-maze test. However, the mechanism by which orally administered l-ornithine reduced the stress response in mice is still unclear. Experiment 1 determined whether orally administered l-ornithine could reduce the stress-induced activation of hypothalamic-pituitary-adrenal axis. Mice were orally administered l-ornithine (0, 0.75, 1.5 and 3. mmol/10. ml/kg, p.o.), and restrained for 30. min from 30. min post administration. There was a significant decrease in the corticosterone levels in the group receiving 0.75. mmol of l-ornithine compared to the control group. In Experiment 2, the effect of orally administered l-ornithine (0 and 0.75. mmol/10. ml/kg, p.o.) on endogenous monoamine release was investigated using in vivo microdialysis. Only the monoamines metabolites 5-hydroxyindoleacetic acid (5-HIAA), dihydroxyphenylacetic acid (DOPAC) and homovallinic acids (HVA) were detected in the present study. Dialysate concentrations of 5-HIAA, DOPAC and HVA were not significantly changed immediately after administration of l-ornithine and restraint stress. In conclusion, changes of corticosterone concentrations by orally administered l-ornithine were not related to alterations in brain monoamine metabolisms.

Original languageEnglish
Pages (from-to)287-291
Number of pages5
JournalNeuroscience Letters
Volume506
Issue number2
DOIs
Publication statusPublished - Jan 11 2012

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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