Oren-gedoku-to and Keishi-bukuryo-gan-ryo inhibit the progression of atherosclerosis in diet-induced hypercholesterolemic rabbits

Nobuyasu Sekiya, Mosaburo Kainuma, Hiroaki Hikiami, Takako Nakagawa, Kazufumi Kouta, Naotoshi Shibahara, Yutaka Shimada, Katsutoshi Terasawa

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

In this study, we examined whether the Kampo formulas Oren-gedoku-to (OGT, Huanglian-jie-du-tang in Chinese) and Keishi-bukuryo-gan-ryo (KBG, Gui-zhi-fu-ling-wan in Chinese) could prevent the progression of atherosclerosis in cholesterol-fed rabbit, an animal model for hypercholesterolemia in vivo. Twenty-four male Japanese white rabbits (2 kg body weight) were divided into four groups. The control group was fed standard rabbit chow containing 1% cholesterol, the OGT group was fed standard rabbit chow containing 1% cholesterol and 1% OGT, the KBG group was fed standard rabbit chow containing 1% cholesterol and 1% KBG, and the vitamin E group was fed standard rabbit chow containing 1% cholesterol and vitamin E (450 mg/1000 g). All four groups were kept on these diets for 8 weeks. At the end of the experiments, the percentage of surface area of the total thoracic aorta with visible plaque was significantly reduced in the OGT and KBG groups. The serum thiobarbituric acid reactive substances of the vitamin E group showed a significantly low value compared with the control group, whereas the serum lipid peroxide levels of the OGT and KBG groups were considerably lower than that of the control groups as well as that of the vitamin E group. Furthermore, the urinary 8-hydroxydeoxyguanosine levels of the OGT and KBG groups were considerably lower than that of the vitamin E group. These results suggest that OGT and KBG prevent the progression of atheromatous plaque by creating a sounder antioxidant defense system than vitamin E.

Original languageEnglish
Pages (from-to)294-298
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume28
Issue number2
DOIs
Publication statusPublished - Feb 1 2005

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Vitamin E
Atherosclerosis
Diet
Rabbits
Cholesterol
Control Groups
Kampo Medicine
Thiobarbituric Acid Reactive Substances
Lipid Peroxides
Atherosclerotic Plaques
Hypercholesterolemia
Serum
Thoracic Aorta
oren gedoku to
keishibukuryogan
Animal Models
Antioxidants
Body Weight

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Oren-gedoku-to and Keishi-bukuryo-gan-ryo inhibit the progression of atherosclerosis in diet-induced hypercholesterolemic rabbits. / Sekiya, Nobuyasu; Kainuma, Mosaburo; Hikiami, Hiroaki; Nakagawa, Takako; Kouta, Kazufumi; Shibahara, Naotoshi; Shimada, Yutaka; Terasawa, Katsutoshi.

In: Biological and Pharmaceutical Bulletin, Vol. 28, No. 2, 01.02.2005, p. 294-298.

Research output: Contribution to journalArticle

Sekiya, Nobuyasu ; Kainuma, Mosaburo ; Hikiami, Hiroaki ; Nakagawa, Takako ; Kouta, Kazufumi ; Shibahara, Naotoshi ; Shimada, Yutaka ; Terasawa, Katsutoshi. / Oren-gedoku-to and Keishi-bukuryo-gan-ryo inhibit the progression of atherosclerosis in diet-induced hypercholesterolemic rabbits. In: Biological and Pharmaceutical Bulletin. 2005 ; Vol. 28, No. 2. pp. 294-298.
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AU - Shimada, Yutaka

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