Orexin-1 receptor immunoreactivity in chemically identified target neurons in the rat hypothalamus

Ryusuke Suzuki; Hisa Shimojima; Hisayuki Funahashi; Shuori Yamada; Jian Lian Guan; Shinobu Tsurugano; Kichiro Uehara; Seiji Shioda; Ryusuke Suzuki; Sakae Kikuyama; Yoichi Takeyama; Hisa Shimojima; Shigeo Nakajo

doi:10.1016/S0304-3940(02)00140-4

Orexin-1 receptor immunoreactivity in chemically identified target neurons in the rat hypothalamus

Ryusuke Suzuki, Hisa Shimojima, Hisayuki Funahashi, Shuori Yamada, Jian Lian Guan, Shinobu Tsurugano, Kichiro Uehara, Seiji Shioda, Ryusuke Suzuki, Sakae Kikuyama, Yoichi Takeyama, Hisa Shimojima, Shigeo Nakajo

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Immunohistochemistry and Western blotting were used to determine the distribution of orexin receptors in the rat brain. Strong orexin receptor 1 (OX₁R) immunoreactivity was detected in the hypothalamus including the arcuate, ventromedial, and tuberomammillary nuclei that are involved in feeding regulation. The neuropeptide Y- and proopiomelanocortin-containing neurons of the arcuate nucleus, which act to stimulate or to inhibit feeding, respectively, displayed intense OX₁R immunoreactivity by double immunostaining. Western blotting analysis yielded a 50-kDa major band of OX₁R.

Original language	English
Pages (from-to)	5-8
Number of pages	4
Journal	Neuroscience Letters
Volume	324
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3940(02)00140-4
Publication status	Published - May 10 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Neuroscience(all)

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10.1016/S0304-3940(02)00140-4

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@article{0c34d5813f0a43f3b0f80c5c3c06fa1f,

title = "Orexin-1 receptor immunoreactivity in chemically identified target neurons in the rat hypothalamus",

abstract = "Immunohistochemistry and Western blotting were used to determine the distribution of orexin receptors in the rat brain. Strong orexin receptor 1 (OX1R) immunoreactivity was detected in the hypothalamus including the arcuate, ventromedial, and tuberomammillary nuclei that are involved in feeding regulation. The neuropeptide Y- and proopiomelanocortin-containing neurons of the arcuate nucleus, which act to stimulate or to inhibit feeding, respectively, displayed intense OX1R immunoreactivity by double immunostaining. Western blotting analysis yielded a 50-kDa major band of OX1R.",

author = "Ryusuke Suzuki and Hisa Shimojima and Hisayuki Funahashi and Shuori Yamada and Guan, {Jian Lian} and Shinobu Tsurugano and Kichiro Uehara and Seiji Shioda and Ryusuke Suzuki and Sakae Kikuyama and Yoichi Takeyama and Hisa Shimojima and Shigeo Nakajo",

note = "Funding Information: The authors wish to thank Ms Sachi Katoh for her technical help. This study was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan (to H.F. and S.S.).",

year = "2002",

month = may,

day = "10",

doi = "10.1016/S0304-3940(02)00140-4",

language = "English",

volume = "324",

pages = "5--8",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "1",

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TY - JOUR

T1 - Orexin-1 receptor immunoreactivity in chemically identified target neurons in the rat hypothalamus

AU - Suzuki, Ryusuke

AU - Shimojima, Hisa

AU - Funahashi, Hisayuki

AU - Yamada, Shuori

AU - Guan, Jian Lian

AU - Tsurugano, Shinobu

AU - Uehara, Kichiro

AU - Shioda, Seiji

AU - Suzuki, Ryusuke

AU - Kikuyama, Sakae

AU - Takeyama, Yoichi

AU - Shimojima, Hisa

AU - Nakajo, Shigeo

N1 - Funding Information: The authors wish to thank Ms Sachi Katoh for her technical help. This study was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan (to H.F. and S.S.).

PY - 2002/5/10

Y1 - 2002/5/10

N2 - Immunohistochemistry and Western blotting were used to determine the distribution of orexin receptors in the rat brain. Strong orexin receptor 1 (OX1R) immunoreactivity was detected in the hypothalamus including the arcuate, ventromedial, and tuberomammillary nuclei that are involved in feeding regulation. The neuropeptide Y- and proopiomelanocortin-containing neurons of the arcuate nucleus, which act to stimulate or to inhibit feeding, respectively, displayed intense OX1R immunoreactivity by double immunostaining. Western blotting analysis yielded a 50-kDa major band of OX1R.

AB - Immunohistochemistry and Western blotting were used to determine the distribution of orexin receptors in the rat brain. Strong orexin receptor 1 (OX1R) immunoreactivity was detected in the hypothalamus including the arcuate, ventromedial, and tuberomammillary nuclei that are involved in feeding regulation. The neuropeptide Y- and proopiomelanocortin-containing neurons of the arcuate nucleus, which act to stimulate or to inhibit feeding, respectively, displayed intense OX1R immunoreactivity by double immunostaining. Western blotting analysis yielded a 50-kDa major band of OX1R.

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DO - 10.1016/S0304-3940(02)00140-4

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JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1

ER -

Orexin-1 receptor immunoreactivity in chemically identified target neurons in the rat hypothalamus

Abstract

All Science Journal Classification (ASJC) codes

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