Orexin-1 receptor immunoreactivity in chemically identified target neurons in the rat hypothalamus

Ryusuke Suzuki, Hisa Shimojima, Hisayuki Funahashi, Shuori Yamada, Jian Lian Guan, Shinobu Tsurugano, Kichiro Uehara, Seiji Shioda, Ryusuke Suzuki, Sakae Kikuyama, Yoichi Takeyama, Hisa Shimojima, Shigeo Nakajo

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Immunohistochemistry and Western blotting were used to determine the distribution of orexin receptors in the rat brain. Strong orexin receptor 1 (OX1R) immunoreactivity was detected in the hypothalamus including the arcuate, ventromedial, and tuberomammillary nuclei that are involved in feeding regulation. The neuropeptide Y- and proopiomelanocortin-containing neurons of the arcuate nucleus, which act to stimulate or to inhibit feeding, respectively, displayed intense OX1R immunoreactivity by double immunostaining. Western blotting analysis yielded a 50-kDa major band of OX1R.

Original languageEnglish
Pages (from-to)5-8
Number of pages4
JournalNeuroscience Letters
Volume324
Issue number1
DOIs
Publication statusPublished - May 10 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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