Organic-Inorganic Hybrid Nanoparticles for Tracking the Same Cells Seamlessly at the Cellular, Tissue, and Whole Body Levels

Koichiro Hayashi, Yusuke Sato, Hiroki Maruoka, Wataru Sakamoto, Toshinobu Yogo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Techniques to elucidate the kinetics and distribution of the same cells in the whole body and in tissues are necessary for further studies of cancer, immunity, and regenerative medicine. Fluorescent imaging is a powerful technique for visualization of cells. However, current fluorescent probes are applicable in either the ultraviolet (UV)-visible (Vis) region (300-650 nm) or the biological transparency window (BTW, 650-900 nm), but not both. Thus, they cannot serve as fluorescent probes for both in vivo and in vitro imaging, and it is difficult to achieve imaging of the same cells seamlessly from the cellular level to the whole body and tissue levels using currently available fluorescent probes. Accordingly, in this paper, we describe organic-inorganic hybrid nanoparticles (HNPs) that could be used to achieve seamless tracking of the same cells. Within the HNPs, a porphyrin molecule, Vis-fluorophore, was surrounded by a siloxane chain, preventing the aggregation of porphyrin molecules. As a result, the porphyrin fluorescence was not quenched. Furthermore, indocyanine green (ICG), a BTW fluorophore, was localized on the HNP surface, leading to fluorescence resonance energy transfer (FRET) from porphyrin to ICG only near the HNP surface. Through the above structural design, the HNPs acquired both excitation (ex) and emission (em) wavelengths in the visible region and BTW, respectively, as well as large Stokes shifts. The HNP-labeled immune cells successfully and the labeled cells were separated easily from unlabeled cells by fluorescence-Activated cell sorting. The kinetics of the labeled cells in the whole body were revealed by fluorescence imaging within BTW. Furthermore, the distributions of the same labeled cells were elucidated by histological analysis within the UV-vis region. Thus, the HNPs served as fluorescent probes for seamless tracking of the same cells.

Original languageEnglish
Pages (from-to)1129-1135
Number of pages7
JournalACS Biomaterials Science and Engineering
Volume3
Issue number6
DOIs
Publication statusPublished - Jun 12 2017
Externally publishedYes

Fingerprint

Tissue
Nanoparticles
Porphyrins
Fluorescent Dyes
Imaging techniques
Indocyanine Green
Fluorophores
Fluorescence
Siloxanes
Molecules
Kinetics
Structural design
Sorting
Transparency
Agglomeration
Visualization
Cells
Wavelength

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Organic-Inorganic Hybrid Nanoparticles for Tracking the Same Cells Seamlessly at the Cellular, Tissue, and Whole Body Levels. / Hayashi, Koichiro; Sato, Yusuke; Maruoka, Hiroki; Sakamoto, Wataru; Yogo, Toshinobu.

In: ACS Biomaterials Science and Engineering, Vol. 3, No. 6, 12.06.2017, p. 1129-1135.

Research output: Contribution to journalArticle

@article{a3e34271275f454a94def8fe877cb893,
title = "Organic-Inorganic Hybrid Nanoparticles for Tracking the Same Cells Seamlessly at the Cellular, Tissue, and Whole Body Levels",
abstract = "Techniques to elucidate the kinetics and distribution of the same cells in the whole body and in tissues are necessary for further studies of cancer, immunity, and regenerative medicine. Fluorescent imaging is a powerful technique for visualization of cells. However, current fluorescent probes are applicable in either the ultraviolet (UV)-visible (Vis) region (300-650 nm) or the biological transparency window (BTW, 650-900 nm), but not both. Thus, they cannot serve as fluorescent probes for both in vivo and in vitro imaging, and it is difficult to achieve imaging of the same cells seamlessly from the cellular level to the whole body and tissue levels using currently available fluorescent probes. Accordingly, in this paper, we describe organic-inorganic hybrid nanoparticles (HNPs) that could be used to achieve seamless tracking of the same cells. Within the HNPs, a porphyrin molecule, Vis-fluorophore, was surrounded by a siloxane chain, preventing the aggregation of porphyrin molecules. As a result, the porphyrin fluorescence was not quenched. Furthermore, indocyanine green (ICG), a BTW fluorophore, was localized on the HNP surface, leading to fluorescence resonance energy transfer (FRET) from porphyrin to ICG only near the HNP surface. Through the above structural design, the HNPs acquired both excitation (ex) and emission (em) wavelengths in the visible region and BTW, respectively, as well as large Stokes shifts. The HNP-labeled immune cells successfully and the labeled cells were separated easily from unlabeled cells by fluorescence-Activated cell sorting. The kinetics of the labeled cells in the whole body were revealed by fluorescence imaging within BTW. Furthermore, the distributions of the same labeled cells were elucidated by histological analysis within the UV-vis region. Thus, the HNPs served as fluorescent probes for seamless tracking of the same cells.",
author = "Koichiro Hayashi and Yusuke Sato and Hiroki Maruoka and Wataru Sakamoto and Toshinobu Yogo",
year = "2017",
month = "6",
day = "12",
doi = "10.1021/acsbiomaterials.7b00181",
language = "English",
volume = "3",
pages = "1129--1135",
journal = "ACS Biomaterials Science and Engineering",
issn = "2373-9878",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Organic-Inorganic Hybrid Nanoparticles for Tracking the Same Cells Seamlessly at the Cellular, Tissue, and Whole Body Levels

AU - Hayashi, Koichiro

AU - Sato, Yusuke

AU - Maruoka, Hiroki

AU - Sakamoto, Wataru

AU - Yogo, Toshinobu

PY - 2017/6/12

Y1 - 2017/6/12

N2 - Techniques to elucidate the kinetics and distribution of the same cells in the whole body and in tissues are necessary for further studies of cancer, immunity, and regenerative medicine. Fluorescent imaging is a powerful technique for visualization of cells. However, current fluorescent probes are applicable in either the ultraviolet (UV)-visible (Vis) region (300-650 nm) or the biological transparency window (BTW, 650-900 nm), but not both. Thus, they cannot serve as fluorescent probes for both in vivo and in vitro imaging, and it is difficult to achieve imaging of the same cells seamlessly from the cellular level to the whole body and tissue levels using currently available fluorescent probes. Accordingly, in this paper, we describe organic-inorganic hybrid nanoparticles (HNPs) that could be used to achieve seamless tracking of the same cells. Within the HNPs, a porphyrin molecule, Vis-fluorophore, was surrounded by a siloxane chain, preventing the aggregation of porphyrin molecules. As a result, the porphyrin fluorescence was not quenched. Furthermore, indocyanine green (ICG), a BTW fluorophore, was localized on the HNP surface, leading to fluorescence resonance energy transfer (FRET) from porphyrin to ICG only near the HNP surface. Through the above structural design, the HNPs acquired both excitation (ex) and emission (em) wavelengths in the visible region and BTW, respectively, as well as large Stokes shifts. The HNP-labeled immune cells successfully and the labeled cells were separated easily from unlabeled cells by fluorescence-Activated cell sorting. The kinetics of the labeled cells in the whole body were revealed by fluorescence imaging within BTW. Furthermore, the distributions of the same labeled cells were elucidated by histological analysis within the UV-vis region. Thus, the HNPs served as fluorescent probes for seamless tracking of the same cells.

AB - Techniques to elucidate the kinetics and distribution of the same cells in the whole body and in tissues are necessary for further studies of cancer, immunity, and regenerative medicine. Fluorescent imaging is a powerful technique for visualization of cells. However, current fluorescent probes are applicable in either the ultraviolet (UV)-visible (Vis) region (300-650 nm) or the biological transparency window (BTW, 650-900 nm), but not both. Thus, they cannot serve as fluorescent probes for both in vivo and in vitro imaging, and it is difficult to achieve imaging of the same cells seamlessly from the cellular level to the whole body and tissue levels using currently available fluorescent probes. Accordingly, in this paper, we describe organic-inorganic hybrid nanoparticles (HNPs) that could be used to achieve seamless tracking of the same cells. Within the HNPs, a porphyrin molecule, Vis-fluorophore, was surrounded by a siloxane chain, preventing the aggregation of porphyrin molecules. As a result, the porphyrin fluorescence was not quenched. Furthermore, indocyanine green (ICG), a BTW fluorophore, was localized on the HNP surface, leading to fluorescence resonance energy transfer (FRET) from porphyrin to ICG only near the HNP surface. Through the above structural design, the HNPs acquired both excitation (ex) and emission (em) wavelengths in the visible region and BTW, respectively, as well as large Stokes shifts. The HNP-labeled immune cells successfully and the labeled cells were separated easily from unlabeled cells by fluorescence-Activated cell sorting. The kinetics of the labeled cells in the whole body were revealed by fluorescence imaging within BTW. Furthermore, the distributions of the same labeled cells were elucidated by histological analysis within the UV-vis region. Thus, the HNPs served as fluorescent probes for seamless tracking of the same cells.

UR - http://www.scopus.com/inward/record.url?scp=85020729180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020729180&partnerID=8YFLogxK

U2 - 10.1021/acsbiomaterials.7b00181

DO - 10.1021/acsbiomaterials.7b00181

M3 - Article

AN - SCOPUS:85020729180

VL - 3

SP - 1129

EP - 1135

JO - ACS Biomaterials Science and Engineering

JF - ACS Biomaterials Science and Engineering

SN - 2373-9878

IS - 6

ER -