Organization and nucleotide sequence of the DNA polymerase gene from the archaeon pyrococcus furiosus

Takashi Uemori, Yoshizumi Ishino, Hiroyuki Toh, Kiyozo Asada, Ikunoshin Kato

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

We cloned the gene encoding the thermostable DNA polymerase from the archaeon Pyrococcus furiosus. The DNA fragment of 2785 base pair (bp) containing the structural gene for DNA polymerase was sequenced. DNA polymerase (Pfu polymerase), as deduced from the DNA sequence, consisted of 775 amlno acids, had a molecular weight of 90,109, and was structurally homologous to the α-like DNA polymerases (family B) represented by human DNA polymerase a and Escherichia coll DNA polymerase II. An unrooted phylogenetic tree of the α-like DNA polymerases based on the amino acid sequence alignment was constructed. Pfu polymerase, with two other archaeon polymerases, constitutes a group with some animal viruses. The transcription initiation sites of the pol gene were identified by analysis of In vivo transcripts of both from P.furiosus and E.coli, and the promoters were assigned upstream of the pol coding region. A typical promoter sequence for the archaeon was found at a reasonable distance from the transcription initiation site in P.furiosus.

Original languageEnglish
Pages (from-to)259-265
Number of pages7
JournalNucleic acids research
Volume21
Issue number2
DOIs
Publication statusPublished - Jan 25 1993

All Science Journal Classification (ASJC) codes

  • Genetics

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