Organization and sequences of the variable, joining and constant region genes of the human T-cell receptor α-chain

Yasunobu Yoshikai, Stephen P. Clark, Sheryle Taylor, Uik Sohn, Bonnie I. Wilson, Mark D. Minden, Tak W. Mak

    Research output: Contribution to journalArticle

    142 Citations (Scopus)

    Abstract

    An essential property of the immune system is its ability to generate great diversity in antibody and T-cell immune responses. The genetic and molecular mechanisms responsible for the generation of antibody diversity have been investigated during the past several years1,2. The gene for the variable (V) region, which determines antigen specificity, is assembled when one member of each of the dispersed clusters of V gene segments, diversity (D) elements (for heavy chains only) and joining (J) segments are fused by DNA rearrangement1-3. The cloning of the β-chain of the T-cell antigen receptor4,5 revealed that the organization of the β-chain locus, which is similar to that of immunoglobulin genes 6-12, is also composed of noncontiguous segments of V7-9, D10,11, J6,9,11 and constant (C) region genes 6-12. The structure of the α-chain seems to consist of a V and a C domain connected by a J segment13-16. We report here that the human T-cell receptor α-chain gene consists of a number of noncontiguous V and J gene segments and a C region gene. The V region gene segment is interrupted by a single intron, whereas the C region contains four exons. The J segments, situated 5′ of the C region gene, are dispersed over a distance of at least 35 kilobases (kb). Signal sequences, which are presumably involved in DNA recombination, are found next to the V and J gene segments.

    Original languageEnglish
    Pages (from-to)837-840
    Number of pages4
    JournalNature
    Volume316
    Issue number6031
    DOIs
    Publication statusPublished - Dec 1 1985

    Fingerprint

    T-Cell Receptor Genes
    Genes
    Antibody Diversity
    T-Lymphocytes
    Immunoglobulin Genes
    Viral Tumor Antigens
    DNA
    Multigene Family
    Protein Sorting Signals
    Introns
    Genetic Recombination
    Organism Cloning
    Molecular Biology
    Exons
    Immune System
    Antigens

    All Science Journal Classification (ASJC) codes

    • Medicine(all)
    • General

    Cite this

    Yoshikai, Y., Clark, S. P., Taylor, S., Sohn, U., Wilson, B. I., Minden, M. D., & Mak, T. W. (1985). Organization and sequences of the variable, joining and constant region genes of the human T-cell receptor α-chain. Nature, 316(6031), 837-840. https://doi.org/10.1038/316837a0

    Organization and sequences of the variable, joining and constant region genes of the human T-cell receptor α-chain. / Yoshikai, Yasunobu; Clark, Stephen P.; Taylor, Sheryle; Sohn, Uik; Wilson, Bonnie I.; Minden, Mark D.; Mak, Tak W.

    In: Nature, Vol. 316, No. 6031, 01.12.1985, p. 837-840.

    Research output: Contribution to journalArticle

    Yoshikai, Y, Clark, SP, Taylor, S, Sohn, U, Wilson, BI, Minden, MD & Mak, TW 1985, 'Organization and sequences of the variable, joining and constant region genes of the human T-cell receptor α-chain', Nature, vol. 316, no. 6031, pp. 837-840. https://doi.org/10.1038/316837a0
    Yoshikai Y, Clark SP, Taylor S, Sohn U, Wilson BI, Minden MD et al. Organization and sequences of the variable, joining and constant region genes of the human T-cell receptor α-chain. Nature. 1985 Dec 1;316(6031):837-840. https://doi.org/10.1038/316837a0
    Yoshikai, Yasunobu ; Clark, Stephen P. ; Taylor, Sheryle ; Sohn, Uik ; Wilson, Bonnie I. ; Minden, Mark D. ; Mak, Tak W. / Organization and sequences of the variable, joining and constant region genes of the human T-cell receptor α-chain. In: Nature. 1985 ; Vol. 316, No. 6031. pp. 837-840.
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