Organization, structure, chromosomal assignment, and expression of the gene encoding the human endothelin-A receptor

K. Hosoda, K. Nakao, N. Tamura, H. Arai, Yoshihiro Ogawa, S. I. Suga, S. Nakanishi, H. Imura

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

We have isolated and characterized the gene for the human endothelin-A receptor. Southern blot analyses demonstrated a single copy gene for the receptor. The gene spans more than 40 kilobases and contains eight exons and seven introns. Intron 1 exists in the 5'-noncoding region, and introns 2-7 occur in the coding region. The locations of introns 2-7 exist before or after the regions encoding the membrane-spanning domains. The transcription start site, determined by primer extension experiments, is 502 base pairs upstream of the methionine initiation codon. The 5'-flanking region lacks a typical TATA box but contains a potential SP-1-binding site 27 base pairs upstream of the transcription start site. Using human-rodent somatic hybrid cell DNA, the gene was assigned to human chromosome 4. Northern blot analyses revealed a 4.3-kilobase mRNA in a wide variety of human tissues, at the highest level in the aorta and at a substantial level in the cultured human mesangial cells. This is the first report of cloning of a gene for a member of the endothelin receptor family. The present study should give a clue to the discovery of possible disorders of the endothelin-A receptor, as well as facilitate the elucidation of the mechanisms by which the gene expression is regulated.

Original languageEnglish
Pages (from-to)18797-18804
Number of pages8
JournalJournal of Biological Chemistry
Volume267
Issue number26
Publication statusPublished - Jan 1 1992
Externally publishedYes

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Endothelin A Receptors
Gene encoding
Genes
Introns
Gene Expression
Transcription Initiation Site
Base Pairing
Endothelin Receptors
TATA Box
Chromosomes, Human, Pair 4
Mesangial Cells
Initiator Codon
Hybrid Cells
5' Flanking Region
Cloning
Human Chromosomes
Chromosomes
Southern Blotting
Gene expression
Northern Blotting

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hosoda, K., Nakao, K., Tamura, N., Arai, H., Ogawa, Y., Suga, S. I., ... Imura, H. (1992). Organization, structure, chromosomal assignment, and expression of the gene encoding the human endothelin-A receptor. Journal of Biological Chemistry, 267(26), 18797-18804.

Organization, structure, chromosomal assignment, and expression of the gene encoding the human endothelin-A receptor. / Hosoda, K.; Nakao, K.; Tamura, N.; Arai, H.; Ogawa, Yoshihiro; Suga, S. I.; Nakanishi, S.; Imura, H.

In: Journal of Biological Chemistry, Vol. 267, No. 26, 01.01.1992, p. 18797-18804.

Research output: Contribution to journalArticle

Hosoda, K, Nakao, K, Tamura, N, Arai, H, Ogawa, Y, Suga, SI, Nakanishi, S & Imura, H 1992, 'Organization, structure, chromosomal assignment, and expression of the gene encoding the human endothelin-A receptor', Journal of Biological Chemistry, vol. 267, no. 26, pp. 18797-18804.
Hosoda, K. ; Nakao, K. ; Tamura, N. ; Arai, H. ; Ogawa, Yoshihiro ; Suga, S. I. ; Nakanishi, S. ; Imura, H. / Organization, structure, chromosomal assignment, and expression of the gene encoding the human endothelin-A receptor. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 26. pp. 18797-18804.
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