Orientation and Order of the Amide Group of Sphingomyelin in Bilayers Determined by Solid-State NMR

Nobuaki Matsumori, Toshiyuki Yamaguchi, Yoshiko Maeta, Michio Murata

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Sphingomyelin (SM) and cholesterol (Chol) are considered essential for the formation of lipid rafts; however, the types of molecular interactions involved in this process, such as intermolecular hydrogen bonding, are not well understood. Since, unlike other phospholipids, SM is characterized by the presence of an amide group, it is essential to determine the orientation of the amide and its order in the lipid bilayers to understand the nature of the hydrogen bonds in lipid rafts. For this study, 1′-13C-2-15N-labeled and 2′-13C-2-15N-labeled SMs were prepared, and the rotational-axis direction and order parameters of the SM amide in bilayers were determined based on 13C and 15N chemical-shift anisotropies and intramolecular 13C-15N dipole coupling constants. Results revealed that the amide orientation was minimally affected by Chol, whereas the order was enhanced significantly in its presence. Thus, Chol likely promotes the formation of an intermolecular hydrogen-bond network involving the SM amide without significantly changing its orientation, providing a higher order to the SM amide. To our knowledge, this study offers new insight into the significance of the SM amide orientation with regard to molecular recognition in lipid rafts, and therefore provides a deeper understanding of the mechanism of their formation.

Original languageEnglish
Pages (from-to)2816-2824
Number of pages9
JournalBiophysical Journal
Volume108
Issue number12
DOIs
Publication statusPublished - Jun 18 2015

All Science Journal Classification (ASJC) codes

  • Biophysics

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