Oriented bone regenerative capacity of octacalcium phosphate/gelatin composites obtained through two-step crystal preparation method

Risa Ishiko-Uzuka, Takahisa Anada, Kazuhito Kobayashi, Tadashi Kawai, Yuji Tanuma, Keiichi Sasaki, Osamu Suzuki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The present study was designed to investigate whether composite of coprecipitating octacalcium phosphate and gelatin (C-OCP/Gel) has an effect in repairing critical-sized defect of rat calvaria with oriented regenerative bone if implanted. The materials were prepared through two steps to disperse homogenous and well-elongated OCP toward long axis of the crystals in gelatin (Gel) matrix with the distinct concentration 17–44 wt %: OCP precipitates recovered from the coprecipitated with Gel molecules in aqueous solution (referred to as C-OCP hereafter) were mixed again in fresh aqueous Gel solution with various mixing ratio to form C-OCP/Gel for implantation. C-OCP/Gel disks with 9 mm diameter and 1 mm thickness after the dehydrothermal treatment was implanted in 9 mm diameter rat calvaria critical-sized defect. The histology, the histomorphometry in the regenerated bone and the quantitative analysis of the orientation of collagen with picrosirius red staining were carried out. It was found that C-OCP/Gel is capable of not only inducing sufficiently regenerative bone over 80% of the defect coupled with practically complete material biodegradation but also forming oriented bone significantly in relation to the amount of C-OCP in Gel matrix until 12 weeks after the implantation.

Original languageEnglish
Pages (from-to)1029-1039
Number of pages11
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume105
Issue number5
DOIs
Publication statusPublished - Jul 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

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