Abstract
The present study was designed to investigate whether composite of coprecipitating octacalcium phosphate and gelatin (C-OCP/Gel) has an effect in repairing critical-sized defect of rat calvaria with oriented regenerative bone if implanted. The materials were prepared through two steps to disperse homogenous and well-elongated OCP toward long axis of the crystals in gelatin (Gel) matrix with the distinct concentration 17–44 wt %: OCP precipitates recovered from the coprecipitated with Gel molecules in aqueous solution (referred to as C-OCP hereafter) were mixed again in fresh aqueous Gel solution with various mixing ratio to form C-OCP/Gel for implantation. C-OCP/Gel disks with 9 mm diameter and 1 mm thickness after the dehydrothermal treatment was implanted in 9 mm diameter rat calvaria critical-sized defect. The histology, the histomorphometry in the regenerated bone and the quantitative analysis of the orientation of collagen with picrosirius red staining were carried out. It was found that C-OCP/Gel is capable of not only inducing sufficiently regenerative bone over 80% of the defect coupled with practically complete material biodegradation but also forming oriented bone significantly in relation to the amount of C-OCP in Gel matrix until 12 weeks after the implantation.
| Original language | English |
|---|---|
| Pages (from-to) | 1029-1039 |
| Number of pages | 11 |
| Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
| Volume | 105 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jul 1 2017 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biomaterials
- Biomedical Engineering
Cite this
Oriented bone regenerative capacity of octacalcium phosphate/gelatin composites obtained through two-step crystal preparation method. / Ishiko-Uzuka, Risa; Anada, Takahisa; Kobayashi, Kazuhito; Kawai, Tadashi; Tanuma, Yuji; Sasaki, Keiichi; Suzuki, Osamu.
In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 105, No. 5, 01.07.2017, p. 1029-1039.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oriented bone regenerative capacity of octacalcium phosphate/gelatin composites obtained through two-step crystal preparation method
AU - Ishiko-Uzuka, Risa
AU - Anada, Takahisa
AU - Kobayashi, Kazuhito
AU - Kawai, Tadashi
AU - Tanuma, Yuji
AU - Sasaki, Keiichi
AU - Suzuki, Osamu
PY - 2017/7/1
Y1 - 2017/7/1
N2 - The present study was designed to investigate whether composite of coprecipitating octacalcium phosphate and gelatin (C-OCP/Gel) has an effect in repairing critical-sized defect of rat calvaria with oriented regenerative bone if implanted. The materials were prepared through two steps to disperse homogenous and well-elongated OCP toward long axis of the crystals in gelatin (Gel) matrix with the distinct concentration 17–44 wt %: OCP precipitates recovered from the coprecipitated with Gel molecules in aqueous solution (referred to as C-OCP hereafter) were mixed again in fresh aqueous Gel solution with various mixing ratio to form C-OCP/Gel for implantation. C-OCP/Gel disks with 9 mm diameter and 1 mm thickness after the dehydrothermal treatment was implanted in 9 mm diameter rat calvaria critical-sized defect. The histology, the histomorphometry in the regenerated bone and the quantitative analysis of the orientation of collagen with picrosirius red staining were carried out. It was found that C-OCP/Gel is capable of not only inducing sufficiently regenerative bone over 80% of the defect coupled with practically complete material biodegradation but also forming oriented bone significantly in relation to the amount of C-OCP in Gel matrix until 12 weeks after the implantation.
AB - The present study was designed to investigate whether composite of coprecipitating octacalcium phosphate and gelatin (C-OCP/Gel) has an effect in repairing critical-sized defect of rat calvaria with oriented regenerative bone if implanted. The materials were prepared through two steps to disperse homogenous and well-elongated OCP toward long axis of the crystals in gelatin (Gel) matrix with the distinct concentration 17–44 wt %: OCP precipitates recovered from the coprecipitated with Gel molecules in aqueous solution (referred to as C-OCP hereafter) were mixed again in fresh aqueous Gel solution with various mixing ratio to form C-OCP/Gel for implantation. C-OCP/Gel disks with 9 mm diameter and 1 mm thickness after the dehydrothermal treatment was implanted in 9 mm diameter rat calvaria critical-sized defect. The histology, the histomorphometry in the regenerated bone and the quantitative analysis of the orientation of collagen with picrosirius red staining were carried out. It was found that C-OCP/Gel is capable of not only inducing sufficiently regenerative bone over 80% of the defect coupled with practically complete material biodegradation but also forming oriented bone significantly in relation to the amount of C-OCP in Gel matrix until 12 weeks after the implantation.
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U2 - 10.1002/jbm.b.33640
DO - 10.1002/jbm.b.33640
M3 - Article
VL - 105
SP - 1029
EP - 1039
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
SN - 1552-4973
IS - 5
ER -