Oriented Helical Peptide Layer on the Carboxylate-Terminated Alkanethiol Immobilized on a Gold Surface

Yoshiko Miura, Shunsaku Kimura, Yukio Imanishi, Junzo Umemura

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Hydrophobic helical hexadecapeptides having an ammonium group were investigated on the formation of an oriented thin layer of the helical peptides on a gold surface. The helical peptides in an ethanol solution were incubated with self-assembled monolayers (SAMs) of carboxylate-terminated alkanethiol on a gold surface to form a helical peptide layer. The thickness of the peptide layer and the orientation of the peptide layer were investigated by the surface plasmon resonance and the FTIR reflection - absorption spectroscopy, respectively. Ion-pair interaction between an ammonium group of the peptides and a carboxylate group of SAMs facilitated the formation of a peptide layer vertically oriented to the substrate. A peptide having an ammonium group and a carboxyl group especially shows a good vertical orientation to the substrate.

Original languageEnglish
Pages (from-to)1155-1160
Number of pages6
JournalLangmuir
Volume15
Issue number4
Publication statusPublished - 1999
Externally publishedYes

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Gold
carboxylates
Peptides
peptides
gold
Ammonium Compounds
Self assembled monolayers
vertical orientation
carboxyl group
Surface plasmon resonance
Substrates
Absorption spectroscopy
surface plasmon resonance
absorption spectroscopy
Ethanol
ethyl alcohol
Ions
ions

All Science Journal Classification (ASJC) codes

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

Oriented Helical Peptide Layer on the Carboxylate-Terminated Alkanethiol Immobilized on a Gold Surface. / Miura, Yoshiko; Kimura, Shunsaku; Imanishi, Yukio; Umemura, Junzo.

In: Langmuir, Vol. 15, No. 4, 1999, p. 1155-1160.

Research output: Contribution to journalArticle

Miura, Y, Kimura, S, Imanishi, Y & Umemura, J 1999, 'Oriented Helical Peptide Layer on the Carboxylate-Terminated Alkanethiol Immobilized on a Gold Surface', Langmuir, vol. 15, no. 4, pp. 1155-1160.
Miura, Yoshiko ; Kimura, Shunsaku ; Imanishi, Yukio ; Umemura, Junzo. / Oriented Helical Peptide Layer on the Carboxylate-Terminated Alkanethiol Immobilized on a Gold Surface. In: Langmuir. 1999 ; Vol. 15, No. 4. pp. 1155-1160.
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