TY - JOUR
T1 - Orthotopic Osteogenecity Enhanced by a Porous Gelatin Sponge in a Critical-Sized Rat Calvaria Defect
AU - Kanda, Naofumi
AU - Anada, Takahisa
AU - Handa, Takuto
AU - Kobayashi, Kazuhito
AU - Ezoe, Yushi
AU - Takahashi, Tetsu
AU - Suzuki, Osamu
N1 - Funding Information:
This study was supported in part by grants in aid (23106010, 26293417, and 15K15720) from the Ministry of Education, Science, Sports, and Culture of Japan.
Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - The gelatin (Gel) powders, derived from acidic and basic extractions of porcine dermis (referred to as AE and BE), were processed for the porous sponge preparation. The disks, which were less than or greater than 500 μm in diameter [small (S) and large (L) pores, respectively] in both extractions and had an interconnected structure respectively, were implanted in critical-sized defects (CSD) of rat calvaria for 4 and 8 weeks to analyze the bone repair capability. Only the AE-S disk induced bone formation (over 60%) histomorphometrically in the CSD after 8 weeks, although the collagen orientation of the regenerated bone was still immature. Osteoblastic cell culture until 14 days did not substantiate marked superiority of AE-S disk regarding the proliferation and the differentiation, although the initial attachment was enhanced on AE-S disk than BE-L disk. The results provide the findings that a Gel sponge with a specific porous structure is capable of inducing orthotopic bone formation in vivo environment. Preparation of gelatin disks for bone regeneration: this study is designed to investigate whether a gelatin sponge with a specific porous structure exhibits orthotopic osteogenecity in critical-sized rat calvarial defects. The results provide the findings that a Gel sponge is capable of inducing new bone formation orthotopically, if the scaffold has a specific porous structure.
AB - The gelatin (Gel) powders, derived from acidic and basic extractions of porcine dermis (referred to as AE and BE), were processed for the porous sponge preparation. The disks, which were less than or greater than 500 μm in diameter [small (S) and large (L) pores, respectively] in both extractions and had an interconnected structure respectively, were implanted in critical-sized defects (CSD) of rat calvaria for 4 and 8 weeks to analyze the bone repair capability. Only the AE-S disk induced bone formation (over 60%) histomorphometrically in the CSD after 8 weeks, although the collagen orientation of the regenerated bone was still immature. Osteoblastic cell culture until 14 days did not substantiate marked superiority of AE-S disk regarding the proliferation and the differentiation, although the initial attachment was enhanced on AE-S disk than BE-L disk. The results provide the findings that a Gel sponge with a specific porous structure is capable of inducing orthotopic bone formation in vivo environment. Preparation of gelatin disks for bone regeneration: this study is designed to investigate whether a gelatin sponge with a specific porous structure exhibits orthotopic osteogenecity in critical-sized rat calvarial defects. The results provide the findings that a Gel sponge is capable of inducing new bone formation orthotopically, if the scaffold has a specific porous structure.
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U2 - 10.1002/mabi.201500191
DO - 10.1002/mabi.201500191
M3 - Article
C2 - 26200698
AN - SCOPUS:84955195501
VL - 15
SP - 1647
EP - 1655
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
SN - 1616-5187
IS - 12
ER -