Oscillation of membrane potential in immobilized DNA membranes

Akon Higuchi, Shinya Adachi, Takeshi Imizu, Yoon Boo Ok, Taro Tsubomura, Mariko Hara, Ken Sakai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Immobilized DNA membranes prepared by binding between DNA and poly(γ-methyl-L-glutamate) membranes having a cis-diaminedichloro platinum group-generated oscillation of the membrane potential under a concentration gradient of several salts at frequencies between 0 and 5 Hertz at pH 7.0, although nonmodified poly(γ-methyl-L-glutamate) membranes showed a constant potential. The amplitude of oscillation depended on the length of the joint segments between the DNA and poly(γ-methyl-L-glutamate) membranes; the immobilized DNA membranes modified with diaminopropane and diaminobutane showed a high oscillation amplitude (e.g., 4-5 mV). It was also influenced by the salts used, and the highest oscillation amplitude in the membrane potential across the immobilized DNA membranes modified with diaminobutane was observed when NaCl was used as the salt solution. Fast Fourier transport analysis revealed that the oscillation of the membrane potential had several specific frequencies depending on the salt solution and the immobilized DNA membranes used. Recognition of the model endocrine disrupters (i.e., dibenzo-p-dioxin and biphenyl) from the oscillation of the membrane potential was performed in the immobilized DNA membranes. The drastic decrease in the membrane potential and the decrease in magnitude of the power spectra were observed in the immobilized DNA membranes modified with hexamethylenediamine after the immobilized DNA membranes were immersed in the saturated solution of dibenzo-p-dioxin. It was qualitatively possible to recognize intercalating molecules of DNA including the endocrine disruptors from the membrane potential oscillation data of the immobilized DNA membranes.

Original languageEnglish
Pages (from-to)9864-9872
Number of pages9
JournalJournal of Physical Chemistry B
Volume104
Issue number42
Publication statusPublished - Oct 26 2000
Externally publishedYes

Fingerprint

Immobilized Nucleic Acids
Membrane Potentials
DNA
deoxyribonucleic acid
membranes
Membranes
oscillations
Salts
Glutamic Acid
1,6-diaminohexane
glutamates
salts
Endocrine Disruptors
Fourier Analysis
Endocrine disrupters
Cisplatin

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Higuchi, A., Adachi, S., Imizu, T., Ok, Y. B., Tsubomura, T., Hara, M., & Sakai, K. (2000). Oscillation of membrane potential in immobilized DNA membranes. Journal of Physical Chemistry B, 104(42), 9864-9872.

Oscillation of membrane potential in immobilized DNA membranes. / Higuchi, Akon; Adachi, Shinya; Imizu, Takeshi; Ok, Yoon Boo; Tsubomura, Taro; Hara, Mariko; Sakai, Ken.

In: Journal of Physical Chemistry B, Vol. 104, No. 42, 26.10.2000, p. 9864-9872.

Research output: Contribution to journalArticle

Higuchi, A, Adachi, S, Imizu, T, Ok, YB, Tsubomura, T, Hara, M & Sakai, K 2000, 'Oscillation of membrane potential in immobilized DNA membranes', Journal of Physical Chemistry B, vol. 104, no. 42, pp. 9864-9872.
Higuchi A, Adachi S, Imizu T, Ok YB, Tsubomura T, Hara M et al. Oscillation of membrane potential in immobilized DNA membranes. Journal of Physical Chemistry B. 2000 Oct 26;104(42):9864-9872.
Higuchi, Akon ; Adachi, Shinya ; Imizu, Takeshi ; Ok, Yoon Boo ; Tsubomura, Taro ; Hara, Mariko ; Sakai, Ken. / Oscillation of membrane potential in immobilized DNA membranes. In: Journal of Physical Chemistry B. 2000 ; Vol. 104, No. 42. pp. 9864-9872.
@article{2cca6517da9743f2b36f90692113249f,
title = "Oscillation of membrane potential in immobilized DNA membranes",
abstract = "Immobilized DNA membranes prepared by binding between DNA and poly(γ-methyl-L-glutamate) membranes having a cis-diaminedichloro platinum group-generated oscillation of the membrane potential under a concentration gradient of several salts at frequencies between 0 and 5 Hertz at pH 7.0, although nonmodified poly(γ-methyl-L-glutamate) membranes showed a constant potential. The amplitude of oscillation depended on the length of the joint segments between the DNA and poly(γ-methyl-L-glutamate) membranes; the immobilized DNA membranes modified with diaminopropane and diaminobutane showed a high oscillation amplitude (e.g., 4-5 mV). It was also influenced by the salts used, and the highest oscillation amplitude in the membrane potential across the immobilized DNA membranes modified with diaminobutane was observed when NaCl was used as the salt solution. Fast Fourier transport analysis revealed that the oscillation of the membrane potential had several specific frequencies depending on the salt solution and the immobilized DNA membranes used. Recognition of the model endocrine disrupters (i.e., dibenzo-p-dioxin and biphenyl) from the oscillation of the membrane potential was performed in the immobilized DNA membranes. The drastic decrease in the membrane potential and the decrease in magnitude of the power spectra were observed in the immobilized DNA membranes modified with hexamethylenediamine after the immobilized DNA membranes were immersed in the saturated solution of dibenzo-p-dioxin. It was qualitatively possible to recognize intercalating molecules of DNA including the endocrine disruptors from the membrane potential oscillation data of the immobilized DNA membranes.",
author = "Akon Higuchi and Shinya Adachi and Takeshi Imizu and Ok, {Yoon Boo} and Taro Tsubomura and Mariko Hara and Ken Sakai",
year = "2000",
month = "10",
day = "26",
language = "English",
volume = "104",
pages = "9864--9872",
journal = "Journal of Physical Chemistry B",
issn = "1520-6106",
number = "42",

}

TY - JOUR

T1 - Oscillation of membrane potential in immobilized DNA membranes

AU - Higuchi, Akon

AU - Adachi, Shinya

AU - Imizu, Takeshi

AU - Ok, Yoon Boo

AU - Tsubomura, Taro

AU - Hara, Mariko

AU - Sakai, Ken

PY - 2000/10/26

Y1 - 2000/10/26

N2 - Immobilized DNA membranes prepared by binding between DNA and poly(γ-methyl-L-glutamate) membranes having a cis-diaminedichloro platinum group-generated oscillation of the membrane potential under a concentration gradient of several salts at frequencies between 0 and 5 Hertz at pH 7.0, although nonmodified poly(γ-methyl-L-glutamate) membranes showed a constant potential. The amplitude of oscillation depended on the length of the joint segments between the DNA and poly(γ-methyl-L-glutamate) membranes; the immobilized DNA membranes modified with diaminopropane and diaminobutane showed a high oscillation amplitude (e.g., 4-5 mV). It was also influenced by the salts used, and the highest oscillation amplitude in the membrane potential across the immobilized DNA membranes modified with diaminobutane was observed when NaCl was used as the salt solution. Fast Fourier transport analysis revealed that the oscillation of the membrane potential had several specific frequencies depending on the salt solution and the immobilized DNA membranes used. Recognition of the model endocrine disrupters (i.e., dibenzo-p-dioxin and biphenyl) from the oscillation of the membrane potential was performed in the immobilized DNA membranes. The drastic decrease in the membrane potential and the decrease in magnitude of the power spectra were observed in the immobilized DNA membranes modified with hexamethylenediamine after the immobilized DNA membranes were immersed in the saturated solution of dibenzo-p-dioxin. It was qualitatively possible to recognize intercalating molecules of DNA including the endocrine disruptors from the membrane potential oscillation data of the immobilized DNA membranes.

AB - Immobilized DNA membranes prepared by binding between DNA and poly(γ-methyl-L-glutamate) membranes having a cis-diaminedichloro platinum group-generated oscillation of the membrane potential under a concentration gradient of several salts at frequencies between 0 and 5 Hertz at pH 7.0, although nonmodified poly(γ-methyl-L-glutamate) membranes showed a constant potential. The amplitude of oscillation depended on the length of the joint segments between the DNA and poly(γ-methyl-L-glutamate) membranes; the immobilized DNA membranes modified with diaminopropane and diaminobutane showed a high oscillation amplitude (e.g., 4-5 mV). It was also influenced by the salts used, and the highest oscillation amplitude in the membrane potential across the immobilized DNA membranes modified with diaminobutane was observed when NaCl was used as the salt solution. Fast Fourier transport analysis revealed that the oscillation of the membrane potential had several specific frequencies depending on the salt solution and the immobilized DNA membranes used. Recognition of the model endocrine disrupters (i.e., dibenzo-p-dioxin and biphenyl) from the oscillation of the membrane potential was performed in the immobilized DNA membranes. The drastic decrease in the membrane potential and the decrease in magnitude of the power spectra were observed in the immobilized DNA membranes modified with hexamethylenediamine after the immobilized DNA membranes were immersed in the saturated solution of dibenzo-p-dioxin. It was qualitatively possible to recognize intercalating molecules of DNA including the endocrine disruptors from the membrane potential oscillation data of the immobilized DNA membranes.

UR - http://www.scopus.com/inward/record.url?scp=0034293936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034293936&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0034293936

VL - 104

SP - 9864

EP - 9872

JO - Journal of Physical Chemistry B

JF - Journal of Physical Chemistry B

SN - 1520-6106

IS - 42

ER -