Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite

Takashi Kumagai, Takahisa Anada, Yoshitomo Honda, Masamichi Takami, Ryutaro Kamijo, Hidetoshi Shimauchi, Osamu Suzuki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.

Original languageEnglish
Pages (from-to)1025-1028
Number of pages4
JournalKey Engineering Materials
Volume361-363 II
Publication statusPublished - Jan 22 2008
Externally publishedYes

Fingerprint

Bone Morphogenetic Protein 2
Durapatite
Hydroxyapatite
Bone
Phosphates
Proteins
Calcium phosphate
Calcium Phosphates
Adsorption
octacalcium phosphate
Temperature
Cells
Plastics
Coatings

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Kumagai, T., Anada, T., Honda, Y., Takami, M., Kamijo, R., Shimauchi, H., & Suzuki, O. (2008). Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite. Key Engineering Materials, 361-363 II, 1025-1028.

Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite. / Kumagai, Takashi; Anada, Takahisa; Honda, Yoshitomo; Takami, Masamichi; Kamijo, Ryutaro; Shimauchi, Hidetoshi; Suzuki, Osamu.

In: Key Engineering Materials, Vol. 361-363 II, 22.01.2008, p. 1025-1028.

Research output: Contribution to journalArticle

Kumagai, T, Anada, T, Honda, Y, Takami, M, Kamijo, R, Shimauchi, H & Suzuki, O 2008, 'Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite', Key Engineering Materials, vol. 361-363 II, pp. 1025-1028.
Kumagai T, Anada T, Honda Y, Takami M, Kamijo R, Shimauchi H et al. Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite. Key Engineering Materials. 2008 Jan 22;361-363 II:1025-1028.
Kumagai, Takashi ; Anada, Takahisa ; Honda, Yoshitomo ; Takami, Masamichi ; Kamijo, Ryutaro ; Shimauchi, Hidetoshi ; Suzuki, Osamu. / Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite. In: Key Engineering Materials. 2008 ; Vol. 361-363 II. pp. 1025-1028.
@article{01b14d7071df49d2890beb36ba5eb15c,
title = "Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite",
abstract = "The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.",
author = "Takashi Kumagai and Takahisa Anada and Yoshitomo Honda and Masamichi Takami and Ryutaro Kamijo and Hidetoshi Shimauchi and Osamu Suzuki",
year = "2008",
month = "1",
day = "22",
language = "English",
volume = "361-363 II",
pages = "1025--1028",
journal = "Key Engineering Materials",
issn = "1013-9826",
publisher = "Trans Tech Publications",

}

TY - JOUR

T1 - Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite

AU - Kumagai, Takashi

AU - Anada, Takahisa

AU - Honda, Yoshitomo

AU - Takami, Masamichi

AU - Kamijo, Ryutaro

AU - Shimauchi, Hidetoshi

AU - Suzuki, Osamu

PY - 2008/1/22

Y1 - 2008/1/22

N2 - The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.

AB - The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.

UR - http://www.scopus.com/inward/record.url?scp=38149104640&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38149104640&partnerID=8YFLogxK

M3 - Article

VL - 361-363 II

SP - 1025

EP - 1028

JO - Key Engineering Materials

JF - Key Engineering Materials

SN - 1013-9826

ER -