Osteoconductive property of a mechanical mixture of octacalcium phosphate and amorphous calcium phosphate

Kazuhito Kobayashi, Takahisa Anada, Takuto Handa, Naofumi Kanda, Mariko Yoshinari, Tetsu Takahashi, Osamu Suzuki

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The present study was designed to investigate the extent of osteoconductive property of a mechanical mixture of octacalcium phosphate (OCP) and amorphous calcium phosphate (ACP). OCP was mixed with ACP in granules that had a diameter of 300 and 500 μm, respectively, and at 25, 50, or 75 wt %. The physicochemical characteristics and the osteoconductive properties of the mixtures were compared with OCP alone or ACP alone through implantation into rat critical-sized calvaria defects for up to 12 weeks and simulated body fluid (SBF) immersion for 2 weeks. The mixtures of OCP and ACP, in particular the OCP 25 wt % and ACP 75 wt % (O25A75), had higher radiopacity compared to ACP and OCP alone. O25A75 induced greater enhancement of bone regeneration than ACP alone at 8 weeks and that than OCP alone at 12 weeks. X-ray diffraction and Fourier transform infrared (FTIR) analyses of the retrieved mixtures showed that ACP, OCP, and O25A75 tended to convert to hydroxyapatite (HA) after the implantation, while the structure of OCP remains without complete conversion after SBF immersion. Analyses by FTIR curve fitting of the solids and the degree of supersaturation of the SBF supported the observation that the existence of ACP enhances the kinetics of the conversion. Scanning electron microscopy found that the surface of O25A75 had distinct characteristics with OCP and ACP after SBF immersion. The results suggest that the extent of the osteoconduction of OCP could be controlled by the copresence of ACP most probably through the prevailing dissolution-precipitation of the surface of ACP crystals to form HA.

Original languageEnglish
Pages (from-to)22602-22611
Number of pages10
JournalACS Applied Materials and Interfaces
Volume6
Issue number24
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Fingerprint

Calcium phosphate
Phosphates
Body fluids
Body Fluids
Immersion
Bone Regeneration
Fourier Analysis
Durapatite
Hydroxyapatite
octacalcium phosphate
amorphous calcium phosphate
Fourier transforms
Infrared radiation
Supersaturation
Curve fitting
Skull
X-Ray Diffraction
Electron Scanning Microscopy
Rats
Bone

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Medicine(all)

Cite this

Osteoconductive property of a mechanical mixture of octacalcium phosphate and amorphous calcium phosphate. / Kobayashi, Kazuhito; Anada, Takahisa; Handa, Takuto; Kanda, Naofumi; Yoshinari, Mariko; Takahashi, Tetsu; Suzuki, Osamu.

In: ACS Applied Materials and Interfaces, Vol. 6, No. 24, 01.01.2014, p. 22602-22611.

Research output: Contribution to journalArticle

Kobayashi, Kazuhito ; Anada, Takahisa ; Handa, Takuto ; Kanda, Naofumi ; Yoshinari, Mariko ; Takahashi, Tetsu ; Suzuki, Osamu. / Osteoconductive property of a mechanical mixture of octacalcium phosphate and amorphous calcium phosphate. In: ACS Applied Materials and Interfaces. 2014 ; Vol. 6, No. 24. pp. 22602-22611.
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