Osteogenesis by gradually expanding the interface between bone surface and periosteum enhanced by bone marrow stem cell administration in rabbits

Koichiro Sato, Naoto Haruyama, Yoshinaka Shimizu, Junichi Hara, Hiroshi Kawamura

Research output: Contribution to journalArticle

27 Citations (Scopus)


Objective: We investigated whether administration of mesenchymal stem cells (MSCs) promotes bone formation at the gap created by periosteal distraction. Study design: A mesh plate was placed subperiosteally in rabbit parietal bones. Following elevation of the mesh plate, rabbit MSCs were administered into the gap. Controls received phosphate-buffered saline (PBS). The volume, height, bone mineral density (BMD), and bone mineral content (BMC) of newly formed bone were examined using microcomputed tomography. Histological analysis was performed by hematoxylin and eosin staining and immunohistochemistry for type I collagen and osteocalcin. Results: The experimental group showed significantly increased volume, height, BMD, and BMC in newly formed bone tissues at the gaps compared with the control group (P <.05). The newly formed bone tissues showed both type I collagen and osteocalcin expression in the MSC-administration group. Conclusion: Mesenchymal stem cell administration may be useful to induce osteogenesis at sites of periosteal distraction.

Original languageEnglish
Pages (from-to)32-40
Number of pages9
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
Issue number1
Publication statusPublished - Mar 1 2010
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • Dentistry(all)

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