Over 50,000-fold sample preconcentration efficiency in microchip electrophoresis using a simple channel

T. Kawai, M. Ueda, K. Sueyoshi, F. Kitagawa, K. Otsuka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Combination of two online sample preconcentration techniques, large-volume sample stacking with an electroosmotic flow pump (LVSEP) and field-amplified sample injection (FASI), was investigated to improve a poor sensitivity of microchip electrophoresis. In the LVSEP-FASI-microchip zone electrophoresis (MCZE) analysis of fluorescein, a 60,000-fold sensitivity increase was achieved both in the single straight channel and Y-channel compared with conventional MCZE. A baseline separation of maltoheptaose (G7) and free dye was also obtained with a 10,000-fold sensitivity increase by LVSEP-FASI in the capillary-based single channel analysis. The relative standard deviations of the detection time and the peak height were 2.4% and 11%, respectively.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages2022-2024
Number of pages3
Publication statusPublished - 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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