Over- and under-expressed microRNAs in human colorectal cancer

Kazuo Motoyama, Hiroshi Inoube, Yasushi Takatsuno, Fumiaki Tanaka, Koshi Mimori, Hiroyuki Uetake, Kenichi Sugihara, Masaki Mori

Research output: Contribution to journalArticle

287 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) constitute a class of small (21-23 nucleotides) noncoding RNAs that function as post-transcriptional gene regulators. It is becoming increasingly clear that altered miRNA expression correlates with the pathogenesis of cancers. The purpose of this study was to determine the up-regulated miRNAs in human colorectal cancer. Total RNA was isolated from cancer tissues and corresponding noncancerous tissues from surgically resected colorectal cancers. The expression profiles of miRNAs were determined using a miRNA microarray containing 455 human miRNA probes. The expression status of selected miRNAs in paired clinical samples was then investigated by real-time RT-PCR. Twenty-one miRNAs were identified by miRNA array analysis as overexpressed in colorectal cancer tissues compared to normal epithelial tissues. Among them, the expression of miR-31, miR-183, miR-17-5p, miR-18a, miR-20a and miR-92 were confirmed to be significantly higher in cancer tissues than in normal tissues (P<0.05). In contrast, the expression of miR-143 and miR-145 in cancer tissues were significantly lower than in normal tissues (P<0.05). The miR-18a overexpression group tended to have a poorer clinical prognosis than the low expression group (P=0.07). We identified miRNAs that were overexpressed or under-expressed in colorectal cancers and which may be correlated with colorectal carcinogenesis.

Original languageEnglish
Pages (from-to)1069-1075
Number of pages7
JournalInternational journal of oncology
Volume34
Issue number4
DOIs
Publication statusPublished - Jul 3 2009

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MicroRNAs
Colorectal Neoplasms
Neoplasms
Untranslated RNA
Regulator Genes
Real-Time Polymerase Chain Reaction
Carcinogenesis
Epithelium
Nucleotides
RNA

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Over- and under-expressed microRNAs in human colorectal cancer. / Motoyama, Kazuo; Inoube, Hiroshi; Takatsuno, Yasushi; Tanaka, Fumiaki; Mimori, Koshi; Uetake, Hiroyuki; Sugihara, Kenichi; Mori, Masaki.

In: International journal of oncology, Vol. 34, No. 4, 03.07.2009, p. 1069-1075.

Research output: Contribution to journalArticle

Motoyama, K, Inoube, H, Takatsuno, Y, Tanaka, F, Mimori, K, Uetake, H, Sugihara, K & Mori, M 2009, 'Over- and under-expressed microRNAs in human colorectal cancer', International journal of oncology, vol. 34, no. 4, pp. 1069-1075. https://doi.org/10.3892/ijo_00000233
Motoyama, Kazuo ; Inoube, Hiroshi ; Takatsuno, Yasushi ; Tanaka, Fumiaki ; Mimori, Koshi ; Uetake, Hiroyuki ; Sugihara, Kenichi ; Mori, Masaki. / Over- and under-expressed microRNAs in human colorectal cancer. In: International journal of oncology. 2009 ; Vol. 34, No. 4. pp. 1069-1075.
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