Overall interaction of cytosolic proteins with the PEI/DNA complex

Takayuki Iida, Takeshi Mori, Yoshiki Katayama, Takuro Niidome

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    Little is known on mechanisms involved in transport of complex of DNA and gene carrier molecules from the cytosol to the nucleus. We aimed to identify cytosolic proteins interacting with the polyethylenimine (PEI)/DNA complex, using 2-D gel electrophoresis and peptide mass fingerprinting. Fifteen proteins including actin, beta-tubulin, and other metabolic proteins were identified. They demonstrated various molecular weights and isoelectric points, and were categorized into 3 groups: early binding, late binding, and transient binding proteins. Protein binding caused DNA release from the PEI/DNA complex with a cation/anion (C/A) ratio of 2, where complex formation was weak. Knowledge on interactions between cytosolic proteins and DNA/carrier complexes will help understand intracellular gene delivery mechanisms.

    Original languageEnglish
    Pages (from-to)364-369
    Number of pages6
    JournalJournal of Controlled Release
    Volume118
    Issue number3
    DOIs
    Publication statusPublished - Apr 23 2007

    All Science Journal Classification (ASJC) codes

    • Pharmaceutical Science

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