Overall interaction of cytosolic proteins with the PEI/DNA complex

Takayuki Iida, Takeshi Mori, Yoshiki Katayama, Takuro Niidome

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Little is known on mechanisms involved in transport of complex of DNA and gene carrier molecules from the cytosol to the nucleus. We aimed to identify cytosolic proteins interacting with the polyethylenimine (PEI)/DNA complex, using 2-D gel electrophoresis and peptide mass fingerprinting. Fifteen proteins including actin, beta-tubulin, and other metabolic proteins were identified. They demonstrated various molecular weights and isoelectric points, and were categorized into 3 groups: early binding, late binding, and transient binding proteins. Protein binding caused DNA release from the PEI/DNA complex with a cation/anion (C/A) ratio of 2, where complex formation was weak. Knowledge on interactions between cytosolic proteins and DNA/carrier complexes will help understand intracellular gene delivery mechanisms.

Original languageEnglish
Pages (from-to)364-369
Number of pages6
JournalJournal of Controlled Release
Volume118
Issue number3
DOIs
Publication statusPublished - Apr 23 2007

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this