Overexpression of 1-Deoxy-d-xylulose-5-phosphate reductoisomerase gene in chloroplast contributes to increment of isoprenoid production

Tomohisa Hasunuma, Shinya Takeno, Shunsuke Hayashi, Mayumi Sendai, Takeshi Bamba, Satomi Yoshimura, Ken Ichi Tomizawa, Eiichiro Fukusaki, Chikahiro Miyake

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Plants synthesize a large number of isoprenoid compounds that are of industrial, nutritional and medicinal importance. 1-Deoxy-d-xylulose reductoisomerase (DXR) catalyzes the first committed step of plastidial isoprenoid-precursor biosynthesis. In the present study, we generated transplastomic tobacco plants that overproduced DXR from Synechosystis sp. strain PCC6803. The transformants showed increase in the content of various isoprenoids such as chlorophyll a, β-carotene, lutein, antheraxanthin, solanesol and β-sitosterol, indicating that the DXR reaction is one of the key steps controlling isoprenoid level in tobacco leaves. A qualitative change in isoprenoid composition was also observed. The growth phenotype of the transplastomic plants was similar to that of wild-type plants. These results showed that plastid metabolic engineering is useful in manipulating the yield of isoprenoids in plants.

Original languageEnglish
Pages (from-to)518-526
Number of pages9
JournalJournal of Bioscience and Bioengineering
Volume105
Issue number5
DOIs
Publication statusPublished - May 1 2008
Externally publishedYes

Fingerprint

Chloroplast Genes
Tobacco
Terpenes
Phosphates
Genes
Xylulose
Metabolic engineering
Biosynthesis
Chlorophyll
Chemical analysis
Metabolic Engineering
Lutein
Plastids
Carotenoids
xylulose-5-phosphate
Phenotype
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Overexpression of 1-Deoxy-d-xylulose-5-phosphate reductoisomerase gene in chloroplast contributes to increment of isoprenoid production. / Hasunuma, Tomohisa; Takeno, Shinya; Hayashi, Shunsuke; Sendai, Mayumi; Bamba, Takeshi; Yoshimura, Satomi; Tomizawa, Ken Ichi; Fukusaki, Eiichiro; Miyake, Chikahiro.

In: Journal of Bioscience and Bioengineering, Vol. 105, No. 5, 01.05.2008, p. 518-526.

Research output: Contribution to journalArticle

Hasunuma, Tomohisa ; Takeno, Shinya ; Hayashi, Shunsuke ; Sendai, Mayumi ; Bamba, Takeshi ; Yoshimura, Satomi ; Tomizawa, Ken Ichi ; Fukusaki, Eiichiro ; Miyake, Chikahiro. / Overexpression of 1-Deoxy-d-xylulose-5-phosphate reductoisomerase gene in chloroplast contributes to increment of isoprenoid production. In: Journal of Bioscience and Bioengineering. 2008 ; Vol. 105, No. 5. pp. 518-526.
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