Overexpression of bcl-2 protein inhibits terminal differentiation of oral keratinocytes in vitro

Hidemitsu Harada, Takeshi Mitsuyasu, Yuji Seta, Yuka Maruoka, Kuniaki Toyoshima, Shigeru Yasumoto

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The bcl-2 proto-oncogene is a known inhibitor of apoptosis; in normal human stratified squamous epithelium, its expression is restricted to the basal cell layer. To investigate the functional role of bcl-2 protein in the process of differentiation of oral keratinocytes, bcl-2 expression vector was transfected into SCC-25 cells, which normally undergo squamous cell differentiation in vitro while expressing specific differentiation markers, e.g., keratin 10/11 and involucrin. In bcl-2 transfected SCC-25 cells, the expression of these differentiation markers was markedly suppressed. The bcl-2 proto-oncogene may play a critical role in opposing the commitment to terminal differentiation and apoptosis of oral keratinocytes.

Original languageEnglish
Pages (from-to)11-17
Number of pages7
JournalJournal of Oral Pathology and Medicine
Volume27
Issue number1
DOIs
Publication statusPublished - Jan 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

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