Overexpression of c-met in the early stage of pancreatic carcinogenesis; altered expression is not sufficient for progression from chronic pancreatitis to pancreatic cancer

Jun Yu, Kenoki Ohuchida, Kazuhiro Mizumoto, Nami Ishikawa, Yasuhiro Ogura, Daisuke Yamada, Takuya Egami, Hayato Fujita, Seiji Ohashi, Eishi Nagai, Masao Tanaka

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Aim: To investigate c-met expression during early pancreatic carcinogenesis. Methods: We used 46 bulk tissues and 36 micro-dissected samples, including normal pancreas, chronic pancreatitis, and pancreatic cancer, for quantitative real-time reverse transcription-polymerase chain reaction. Results: In bulk tissue analyses, pancreatic cancer tissues expressed significantly higher levels of c-met than did chronic pancreatitis and normal pancreas tissues. c-met levels did not differ between chronic pancreatitis and normal pancreas tissues. In microdissection-based analyses, c-met was expressed at higher levels in microdissected pancreatic cancer cells and pancreatitis-affected epithelial cells than in normal ductal epithelial cells (both, P < 0.01). Interestingly, pancreatitis-affected epithelial cells expressed levels of c-met similar to those of pancreatic cancer cells. Conclusion: Overexpression of c-met occurs during the early stage of pancreatic carcinogenesis, and a single alteration of c-met expression is not sufficient for progression of chronic pancreatitis-affected epithelial cells to pancreatic cancer cells.

Original languageEnglish
Pages (from-to)3878-3882
Number of pages5
JournalWorld Journal of Gastroenterology
Volume12
Issue number24
DOIs
Publication statusPublished - Jun 28 2006

Fingerprint

Chronic Pancreatitis
Pancreatic Neoplasms
Carcinogenesis
Epithelial Cells
Pancreas
Pancreatitis
Microdissection
Reverse Transcription
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

Overexpression of c-met in the early stage of pancreatic carcinogenesis; altered expression is not sufficient for progression from chronic pancreatitis to pancreatic cancer. / Yu, Jun; Ohuchida, Kenoki; Mizumoto, Kazuhiro; Ishikawa, Nami; Ogura, Yasuhiro; Yamada, Daisuke; Egami, Takuya; Fujita, Hayato; Ohashi, Seiji; Nagai, Eishi; Tanaka, Masao.

In: World Journal of Gastroenterology, Vol. 12, No. 24, 28.06.2006, p. 3878-3882.

Research output: Contribution to journalArticle

@article{e3ba2219e34c4acaa61932cafb27e165,
title = "Overexpression of c-met in the early stage of pancreatic carcinogenesis; altered expression is not sufficient for progression from chronic pancreatitis to pancreatic cancer",
abstract = "Aim: To investigate c-met expression during early pancreatic carcinogenesis. Methods: We used 46 bulk tissues and 36 micro-dissected samples, including normal pancreas, chronic pancreatitis, and pancreatic cancer, for quantitative real-time reverse transcription-polymerase chain reaction. Results: In bulk tissue analyses, pancreatic cancer tissues expressed significantly higher levels of c-met than did chronic pancreatitis and normal pancreas tissues. c-met levels did not differ between chronic pancreatitis and normal pancreas tissues. In microdissection-based analyses, c-met was expressed at higher levels in microdissected pancreatic cancer cells and pancreatitis-affected epithelial cells than in normal ductal epithelial cells (both, P < 0.01). Interestingly, pancreatitis-affected epithelial cells expressed levels of c-met similar to those of pancreatic cancer cells. Conclusion: Overexpression of c-met occurs during the early stage of pancreatic carcinogenesis, and a single alteration of c-met expression is not sufficient for progression of chronic pancreatitis-affected epithelial cells to pancreatic cancer cells.",
author = "Jun Yu and Kenoki Ohuchida and Kazuhiro Mizumoto and Nami Ishikawa and Yasuhiro Ogura and Daisuke Yamada and Takuya Egami and Hayato Fujita and Seiji Ohashi and Eishi Nagai and Masao Tanaka",
year = "2006",
month = "6",
day = "28",
doi = "10.3748/wjg.v12.i24.3878",
language = "English",
volume = "12",
pages = "3878--3882",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "24",

}

TY - JOUR

T1 - Overexpression of c-met in the early stage of pancreatic carcinogenesis; altered expression is not sufficient for progression from chronic pancreatitis to pancreatic cancer

AU - Yu, Jun

AU - Ohuchida, Kenoki

AU - Mizumoto, Kazuhiro

AU - Ishikawa, Nami

AU - Ogura, Yasuhiro

AU - Yamada, Daisuke

AU - Egami, Takuya

AU - Fujita, Hayato

AU - Ohashi, Seiji

AU - Nagai, Eishi

AU - Tanaka, Masao

PY - 2006/6/28

Y1 - 2006/6/28

N2 - Aim: To investigate c-met expression during early pancreatic carcinogenesis. Methods: We used 46 bulk tissues and 36 micro-dissected samples, including normal pancreas, chronic pancreatitis, and pancreatic cancer, for quantitative real-time reverse transcription-polymerase chain reaction. Results: In bulk tissue analyses, pancreatic cancer tissues expressed significantly higher levels of c-met than did chronic pancreatitis and normal pancreas tissues. c-met levels did not differ between chronic pancreatitis and normal pancreas tissues. In microdissection-based analyses, c-met was expressed at higher levels in microdissected pancreatic cancer cells and pancreatitis-affected epithelial cells than in normal ductal epithelial cells (both, P < 0.01). Interestingly, pancreatitis-affected epithelial cells expressed levels of c-met similar to those of pancreatic cancer cells. Conclusion: Overexpression of c-met occurs during the early stage of pancreatic carcinogenesis, and a single alteration of c-met expression is not sufficient for progression of chronic pancreatitis-affected epithelial cells to pancreatic cancer cells.

AB - Aim: To investigate c-met expression during early pancreatic carcinogenesis. Methods: We used 46 bulk tissues and 36 micro-dissected samples, including normal pancreas, chronic pancreatitis, and pancreatic cancer, for quantitative real-time reverse transcription-polymerase chain reaction. Results: In bulk tissue analyses, pancreatic cancer tissues expressed significantly higher levels of c-met than did chronic pancreatitis and normal pancreas tissues. c-met levels did not differ between chronic pancreatitis and normal pancreas tissues. In microdissection-based analyses, c-met was expressed at higher levels in microdissected pancreatic cancer cells and pancreatitis-affected epithelial cells than in normal ductal epithelial cells (both, P < 0.01). Interestingly, pancreatitis-affected epithelial cells expressed levels of c-met similar to those of pancreatic cancer cells. Conclusion: Overexpression of c-met occurs during the early stage of pancreatic carcinogenesis, and a single alteration of c-met expression is not sufficient for progression of chronic pancreatitis-affected epithelial cells to pancreatic cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=33745853820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745853820&partnerID=8YFLogxK

U2 - 10.3748/wjg.v12.i24.3878

DO - 10.3748/wjg.v12.i24.3878

M3 - Article

C2 - 16804974

AN - SCOPUS:33745853820

VL - 12

SP - 3878

EP - 3882

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 24

ER -