Overexpression of CD163 in vitreous and fibrovascular membranes of patients with proliferative diabetic retinopathy: Possible involvement of periostin

Yoshiyuki Kobayashi, Shigeo Yoshida, Takahito Nakama, Yedi Zhou, Keijiro Ishikawa, Ryoichi Arita, Shintaro Nakao, Masanori Miyazaki, Yukio Sassa, Yuji Oshima, Kenji Izuhara, Toshihiro Kono, Tatsuro Ishibashi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aim To determine whether CD163, a specific marker for M2 macrophages, is involved in the formation of preretinal fibrovascular membranes (FVMs) present in eyes with proliferative diabetic retinopathy (PDR). Methods We measured the levels of soluble (s)CD163, periostin and vascular endothelial growth factor by sandwich ELISA in vitreous samples from 74 eyes of 62 patients with PDR, 20 eyes of 18 patients with proliferative vitreoretinopathy, and 56 eyes of 54 patients with non-diabetic ocular diseases (control group). Immunohistochemical analyses were performed to determine the expressions of CD68, CD163 and periostin in the surgically resected FVMs and idiopathic epiretinal membranes (ERMs). Results The concentrations of sCD163 and periostin in the vitreous were significantly higher in patients with PDR than in non-diabetic controls (p<0.0001). There was a strong correlation between the vitreous concentrations of sCD163 and periostin. The mean vitreous level of sCD163 was significantly higher in eyes with FVMs than in those without FVMs (epicentre only). The number and percentage of CD163+ macrophages were significantly higher in the FVMs than in the idiopathic ERMs. Immunohistochemical analysis showed co-localisation of CD163 and periostin in FVM cells. Conclusions These findings indicate that the overexpression of CD163 by macrophages may be involved in the development of FVMs partly through periostin production.

Original languageEnglish
Pages (from-to)451-456
Number of pages6
JournalBritish Journal of Ophthalmology
Volume99
Issue number4
DOIs
Publication statusPublished - Apr 1 2015

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Diabetic Retinopathy
Membranes
Epiretinal Membrane
Macrophages
Proliferative Vitreoretinopathy
Eye Diseases
Vascular Endothelial Growth Factor A
Enzyme-Linked Immunosorbent Assay
Cell Membrane
Control Groups

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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Overexpression of CD163 in vitreous and fibrovascular membranes of patients with proliferative diabetic retinopathy : Possible involvement of periostin. / Kobayashi, Yoshiyuki; Yoshida, Shigeo; Nakama, Takahito; Zhou, Yedi; Ishikawa, Keijiro; Arita, Ryoichi; Nakao, Shintaro; Miyazaki, Masanori; Sassa, Yukio; Oshima, Yuji; Izuhara, Kenji; Kono, Toshihiro; Ishibashi, Tatsuro.

In: British Journal of Ophthalmology, Vol. 99, No. 4, 01.04.2015, p. 451-456.

Research output: Contribution to journalArticle

Kobayashi, Yoshiyuki ; Yoshida, Shigeo ; Nakama, Takahito ; Zhou, Yedi ; Ishikawa, Keijiro ; Arita, Ryoichi ; Nakao, Shintaro ; Miyazaki, Masanori ; Sassa, Yukio ; Oshima, Yuji ; Izuhara, Kenji ; Kono, Toshihiro ; Ishibashi, Tatsuro. / Overexpression of CD163 in vitreous and fibrovascular membranes of patients with proliferative diabetic retinopathy : Possible involvement of periostin. In: British Journal of Ophthalmology. 2015 ; Vol. 99, No. 4. pp. 451-456.
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abstract = "Aim To determine whether CD163, a specific marker for M2 macrophages, is involved in the formation of preretinal fibrovascular membranes (FVMs) present in eyes with proliferative diabetic retinopathy (PDR). Methods We measured the levels of soluble (s)CD163, periostin and vascular endothelial growth factor by sandwich ELISA in vitreous samples from 74 eyes of 62 patients with PDR, 20 eyes of 18 patients with proliferative vitreoretinopathy, and 56 eyes of 54 patients with non-diabetic ocular diseases (control group). Immunohistochemical analyses were performed to determine the expressions of CD68, CD163 and periostin in the surgically resected FVMs and idiopathic epiretinal membranes (ERMs). Results The concentrations of sCD163 and periostin in the vitreous were significantly higher in patients with PDR than in non-diabetic controls (p<0.0001). There was a strong correlation between the vitreous concentrations of sCD163 and periostin. The mean vitreous level of sCD163 was significantly higher in eyes with FVMs than in those without FVMs (epicentre only). The number and percentage of CD163+ macrophages were significantly higher in the FVMs than in the idiopathic ERMs. Immunohistochemical analysis showed co-localisation of CD163 and periostin in FVM cells. Conclusions These findings indicate that the overexpression of CD163 by macrophages may be involved in the development of FVMs partly through periostin production.",
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T1 - Overexpression of CD163 in vitreous and fibrovascular membranes of patients with proliferative diabetic retinopathy

T2 - Possible involvement of periostin

AU - Kobayashi, Yoshiyuki

AU - Yoshida, Shigeo

AU - Nakama, Takahito

AU - Zhou, Yedi

AU - Ishikawa, Keijiro

AU - Arita, Ryoichi

AU - Nakao, Shintaro

AU - Miyazaki, Masanori

AU - Sassa, Yukio

AU - Oshima, Yuji

AU - Izuhara, Kenji

AU - Kono, Toshihiro

AU - Ishibashi, Tatsuro

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Aim To determine whether CD163, a specific marker for M2 macrophages, is involved in the formation of preretinal fibrovascular membranes (FVMs) present in eyes with proliferative diabetic retinopathy (PDR). Methods We measured the levels of soluble (s)CD163, periostin and vascular endothelial growth factor by sandwich ELISA in vitreous samples from 74 eyes of 62 patients with PDR, 20 eyes of 18 patients with proliferative vitreoretinopathy, and 56 eyes of 54 patients with non-diabetic ocular diseases (control group). Immunohistochemical analyses were performed to determine the expressions of CD68, CD163 and periostin in the surgically resected FVMs and idiopathic epiretinal membranes (ERMs). Results The concentrations of sCD163 and periostin in the vitreous were significantly higher in patients with PDR than in non-diabetic controls (p<0.0001). There was a strong correlation between the vitreous concentrations of sCD163 and periostin. The mean vitreous level of sCD163 was significantly higher in eyes with FVMs than in those without FVMs (epicentre only). The number and percentage of CD163+ macrophages were significantly higher in the FVMs than in the idiopathic ERMs. Immunohistochemical analysis showed co-localisation of CD163 and periostin in FVM cells. Conclusions These findings indicate that the overexpression of CD163 by macrophages may be involved in the development of FVMs partly through periostin production.

AB - Aim To determine whether CD163, a specific marker for M2 macrophages, is involved in the formation of preretinal fibrovascular membranes (FVMs) present in eyes with proliferative diabetic retinopathy (PDR). Methods We measured the levels of soluble (s)CD163, periostin and vascular endothelial growth factor by sandwich ELISA in vitreous samples from 74 eyes of 62 patients with PDR, 20 eyes of 18 patients with proliferative vitreoretinopathy, and 56 eyes of 54 patients with non-diabetic ocular diseases (control group). Immunohistochemical analyses were performed to determine the expressions of CD68, CD163 and periostin in the surgically resected FVMs and idiopathic epiretinal membranes (ERMs). Results The concentrations of sCD163 and periostin in the vitreous were significantly higher in patients with PDR than in non-diabetic controls (p<0.0001). There was a strong correlation between the vitreous concentrations of sCD163 and periostin. The mean vitreous level of sCD163 was significantly higher in eyes with FVMs than in those without FVMs (epicentre only). The number and percentage of CD163+ macrophages were significantly higher in the FVMs than in the idiopathic ERMs. Immunohistochemical analysis showed co-localisation of CD163 and periostin in FVM cells. Conclusions These findings indicate that the overexpression of CD163 by macrophages may be involved in the development of FVMs partly through periostin production.

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