Overexpression of cyclooxygenase-2 and tumor angiogenesis in human gastric cancer

Tadashi Koga, Kotaro Shibahara, Akira Kabashima, Yasushi Sumiyoshi, Yasue Kimura, Ikuo Takahashi, Yoshihiro Kakeji, Yoshihiko Maehara

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background/Aims: Cyclooxygenase-2 (COX-2) protein is overexpressed in various cancers, including esophageal, gastric, colon, and pancreatic. To better comprehend the role of COX-2 in gastric cancer, especially with regard to angiogenesis, we investigated COX-2 and vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in 108 patients with gastric cancer. Methodology: We used immunohistochemical analysis of formalin-fixed tissues of gastric cancer. Results: Expression of COX-2 showed diffuse staining in the cytoplasm of tumor cells, however, no staining in normal epithelial cells. Of the 108 tumors examined, 71 (65%) were positive for COX-2 expression, the VEGF-positive cases numbered 43 of 108 cases (39.8%). The intensity of COX-2 expression did not correlate with any clinicopathological characteristics. The positive rate of VEGF expression in COX-2-positive cases was significantly higher than in COX-2-negative ones (47.9% vs. 24.3%, P < 0.05). MVD in COX-2-positive cases was significantly higher than in COX-2-negative ones (22.0 ± 7.8 vs. 18.5 ± 7.5/1mm2; P < 0.05). Conclusions: Our study provides evidence that COX-2 is closely related with angiogenesis.

Original languageEnglish
Pages (from-to)1626-1630
Number of pages5
JournalHepato-Gastroenterology
Volume51
Issue number60
Publication statusPublished - Nov 1 2004

Fingerprint

Cyclooxygenase 2
Stomach Neoplasms
Neoplasms
Vascular Endothelial Growth Factor A
Microvessels
Staining and Labeling
Esophageal Neoplasms
Formaldehyde
Stomach
Colon
Cytoplasm
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Koga, T., Shibahara, K., Kabashima, A., Sumiyoshi, Y., Kimura, Y., Takahashi, I., ... Maehara, Y. (2004). Overexpression of cyclooxygenase-2 and tumor angiogenesis in human gastric cancer. Hepato-Gastroenterology, 51(60), 1626-1630.

Overexpression of cyclooxygenase-2 and tumor angiogenesis in human gastric cancer. / Koga, Tadashi; Shibahara, Kotaro; Kabashima, Akira; Sumiyoshi, Yasushi; Kimura, Yasue; Takahashi, Ikuo; Kakeji, Yoshihiro; Maehara, Yoshihiko.

In: Hepato-Gastroenterology, Vol. 51, No. 60, 01.11.2004, p. 1626-1630.

Research output: Contribution to journalArticle

Koga, T, Shibahara, K, Kabashima, A, Sumiyoshi, Y, Kimura, Y, Takahashi, I, Kakeji, Y & Maehara, Y 2004, 'Overexpression of cyclooxygenase-2 and tumor angiogenesis in human gastric cancer', Hepato-Gastroenterology, vol. 51, no. 60, pp. 1626-1630.
Koga T, Shibahara K, Kabashima A, Sumiyoshi Y, Kimura Y, Takahashi I et al. Overexpression of cyclooxygenase-2 and tumor angiogenesis in human gastric cancer. Hepato-Gastroenterology. 2004 Nov 1;51(60):1626-1630.
Koga, Tadashi ; Shibahara, Kotaro ; Kabashima, Akira ; Sumiyoshi, Yasushi ; Kimura, Yasue ; Takahashi, Ikuo ; Kakeji, Yoshihiro ; Maehara, Yoshihiko. / Overexpression of cyclooxygenase-2 and tumor angiogenesis in human gastric cancer. In: Hepato-Gastroenterology. 2004 ; Vol. 51, No. 60. pp. 1626-1630.
@article{c1bc9e208b734fc08e915125c2aedb06,
title = "Overexpression of cyclooxygenase-2 and tumor angiogenesis in human gastric cancer",
abstract = "Background/Aims: Cyclooxygenase-2 (COX-2) protein is overexpressed in various cancers, including esophageal, gastric, colon, and pancreatic. To better comprehend the role of COX-2 in gastric cancer, especially with regard to angiogenesis, we investigated COX-2 and vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in 108 patients with gastric cancer. Methodology: We used immunohistochemical analysis of formalin-fixed tissues of gastric cancer. Results: Expression of COX-2 showed diffuse staining in the cytoplasm of tumor cells, however, no staining in normal epithelial cells. Of the 108 tumors examined, 71 (65{\%}) were positive for COX-2 expression, the VEGF-positive cases numbered 43 of 108 cases (39.8{\%}). The intensity of COX-2 expression did not correlate with any clinicopathological characteristics. The positive rate of VEGF expression in COX-2-positive cases was significantly higher than in COX-2-negative ones (47.9{\%} vs. 24.3{\%}, P < 0.05). MVD in COX-2-positive cases was significantly higher than in COX-2-negative ones (22.0 ± 7.8 vs. 18.5 ± 7.5/1mm2; P < 0.05). Conclusions: Our study provides evidence that COX-2 is closely related with angiogenesis.",
author = "Tadashi Koga and Kotaro Shibahara and Akira Kabashima and Yasushi Sumiyoshi and Yasue Kimura and Ikuo Takahashi and Yoshihiro Kakeji and Yoshihiko Maehara",
year = "2004",
month = "11",
day = "1",
language = "English",
volume = "51",
pages = "1626--1630",
journal = "Acta hepato-splenologica",
issn = "0172-6390",
publisher = "H.G.E. Update Medical Publishing Ltd.",
number = "60",

}

TY - JOUR

T1 - Overexpression of cyclooxygenase-2 and tumor angiogenesis in human gastric cancer

AU - Koga, Tadashi

AU - Shibahara, Kotaro

AU - Kabashima, Akira

AU - Sumiyoshi, Yasushi

AU - Kimura, Yasue

AU - Takahashi, Ikuo

AU - Kakeji, Yoshihiro

AU - Maehara, Yoshihiko

PY - 2004/11/1

Y1 - 2004/11/1

N2 - Background/Aims: Cyclooxygenase-2 (COX-2) protein is overexpressed in various cancers, including esophageal, gastric, colon, and pancreatic. To better comprehend the role of COX-2 in gastric cancer, especially with regard to angiogenesis, we investigated COX-2 and vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in 108 patients with gastric cancer. Methodology: We used immunohistochemical analysis of formalin-fixed tissues of gastric cancer. Results: Expression of COX-2 showed diffuse staining in the cytoplasm of tumor cells, however, no staining in normal epithelial cells. Of the 108 tumors examined, 71 (65%) were positive for COX-2 expression, the VEGF-positive cases numbered 43 of 108 cases (39.8%). The intensity of COX-2 expression did not correlate with any clinicopathological characteristics. The positive rate of VEGF expression in COX-2-positive cases was significantly higher than in COX-2-negative ones (47.9% vs. 24.3%, P < 0.05). MVD in COX-2-positive cases was significantly higher than in COX-2-negative ones (22.0 ± 7.8 vs. 18.5 ± 7.5/1mm2; P < 0.05). Conclusions: Our study provides evidence that COX-2 is closely related with angiogenesis.

AB - Background/Aims: Cyclooxygenase-2 (COX-2) protein is overexpressed in various cancers, including esophageal, gastric, colon, and pancreatic. To better comprehend the role of COX-2 in gastric cancer, especially with regard to angiogenesis, we investigated COX-2 and vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in 108 patients with gastric cancer. Methodology: We used immunohistochemical analysis of formalin-fixed tissues of gastric cancer. Results: Expression of COX-2 showed diffuse staining in the cytoplasm of tumor cells, however, no staining in normal epithelial cells. Of the 108 tumors examined, 71 (65%) were positive for COX-2 expression, the VEGF-positive cases numbered 43 of 108 cases (39.8%). The intensity of COX-2 expression did not correlate with any clinicopathological characteristics. The positive rate of VEGF expression in COX-2-positive cases was significantly higher than in COX-2-negative ones (47.9% vs. 24.3%, P < 0.05). MVD in COX-2-positive cases was significantly higher than in COX-2-negative ones (22.0 ± 7.8 vs. 18.5 ± 7.5/1mm2; P < 0.05). Conclusions: Our study provides evidence that COX-2 is closely related with angiogenesis.

UR - http://www.scopus.com/inward/record.url?scp=6344222880&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=6344222880&partnerID=8YFLogxK

M3 - Article

VL - 51

SP - 1626

EP - 1630

JO - Acta hepato-splenologica

JF - Acta hepato-splenologica

SN - 0172-6390

IS - 60

ER -