Overexpression of eNOS in RVLM improves impaired baroreflex control of heart rate in SHRSP

Takuya Kishi, Yoshitaka Hirooka, Yoshikuni Kimura, Koji Sakai, Koji Ito, Hiroaki Shimokawa, Akira Takeshita

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

We previously demonstrated that the overexpression of endothelial nitric oxide synthase (eNOS) in the rostral ventrolateral medulla (RVLM) decreases blood pressure, heart rate (HR), and sympathetic nerve activity and that these effects are enhanced in stroke-prone spontaneously hypertensive rats (SHRSP). The aim of this study was to determine if an increase in NO production in the RVLM caused by the overexpression of eNOS improves the impaired baroreflex control of HR in SHRSP. We transfected adenovirus vectors encoding eNOS (AdeNOS) into the RVLM of SHRSP or Wistar-Kyoto rats (WKY). Mean arterial pressure and HR were measured by a radio-telemetry system in the conscious state. Reflex changes in HR were elicited by intravenous infusion of either phenylephrine, sodium nitroprusside, or hydralazine at day 7 after the gene transfer. The maximum gain of the baroreflex control of HR was significantly decreased in SHRSP compared with WKY. Overexpression of eNOS in the RVLM of SHRSP improved the impaired maximum gain of the baroreflex control of HR. After treatment with atropine, the maximum gain was still significantly greater in SHRSP in the AdeNOS-transfected group than in the nontransfected group, although it was decreased in both groups. In contrast, after treatment with metoprolol, the maximum gain did not differ between the two groups. These results indicate that an increase in NO production in the RVLM improves the impaired baroreflex control of HR in SHRSP and that these effects may have resulted from a cardiac sympathoinhibitory effect of NO in the RVLM of SHRSP.

Original languageEnglish
Pages (from-to)255-260
Number of pages6
JournalHypertension
Volume41
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

Fingerprint

Baroreflex
Nitric Oxide Synthase Type III
Heart Rate
Inbred WKY Rats
Adenoviridae
Hydralazine
Telemetry
Metoprolol
Nitroprusside
Phenylephrine
Inbred SHR Rats
Radio
Atropine
Intravenous Infusions
Reflex
Arterial Pressure
Stroke
Blood Pressure
Therapeutics
Genes

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Kishi, T., Hirooka, Y., Kimura, Y., Sakai, K., Ito, K., Shimokawa, H., & Takeshita, A. (2003). Overexpression of eNOS in RVLM improves impaired baroreflex control of heart rate in SHRSP. Hypertension, 41(2), 255-260. https://doi.org/10.1161/01.HYP.0000050649.30821.CB

Overexpression of eNOS in RVLM improves impaired baroreflex control of heart rate in SHRSP. / Kishi, Takuya; Hirooka, Yoshitaka; Kimura, Yoshikuni; Sakai, Koji; Ito, Koji; Shimokawa, Hiroaki; Takeshita, Akira.

In: Hypertension, Vol. 41, No. 2, 01.02.2003, p. 255-260.

Research output: Contribution to journalArticle

Kishi, T, Hirooka, Y, Kimura, Y, Sakai, K, Ito, K, Shimokawa, H & Takeshita, A 2003, 'Overexpression of eNOS in RVLM improves impaired baroreflex control of heart rate in SHRSP', Hypertension, vol. 41, no. 2, pp. 255-260. https://doi.org/10.1161/01.HYP.0000050649.30821.CB
Kishi, Takuya ; Hirooka, Yoshitaka ; Kimura, Yoshikuni ; Sakai, Koji ; Ito, Koji ; Shimokawa, Hiroaki ; Takeshita, Akira. / Overexpression of eNOS in RVLM improves impaired baroreflex control of heart rate in SHRSP. In: Hypertension. 2003 ; Vol. 41, No. 2. pp. 255-260.
@article{42061b618d7c44da83f6f49909c8c555,
title = "Overexpression of eNOS in RVLM improves impaired baroreflex control of heart rate in SHRSP",
abstract = "We previously demonstrated that the overexpression of endothelial nitric oxide synthase (eNOS) in the rostral ventrolateral medulla (RVLM) decreases blood pressure, heart rate (HR), and sympathetic nerve activity and that these effects are enhanced in stroke-prone spontaneously hypertensive rats (SHRSP). The aim of this study was to determine if an increase in NO production in the RVLM caused by the overexpression of eNOS improves the impaired baroreflex control of HR in SHRSP. We transfected adenovirus vectors encoding eNOS (AdeNOS) into the RVLM of SHRSP or Wistar-Kyoto rats (WKY). Mean arterial pressure and HR were measured by a radio-telemetry system in the conscious state. Reflex changes in HR were elicited by intravenous infusion of either phenylephrine, sodium nitroprusside, or hydralazine at day 7 after the gene transfer. The maximum gain of the baroreflex control of HR was significantly decreased in SHRSP compared with WKY. Overexpression of eNOS in the RVLM of SHRSP improved the impaired maximum gain of the baroreflex control of HR. After treatment with atropine, the maximum gain was still significantly greater in SHRSP in the AdeNOS-transfected group than in the nontransfected group, although it was decreased in both groups. In contrast, after treatment with metoprolol, the maximum gain did not differ between the two groups. These results indicate that an increase in NO production in the RVLM improves the impaired baroreflex control of HR in SHRSP and that these effects may have resulted from a cardiac sympathoinhibitory effect of NO in the RVLM of SHRSP.",
author = "Takuya Kishi and Yoshitaka Hirooka and Yoshikuni Kimura and Koji Sakai and Koji Ito and Hiroaki Shimokawa and Akira Takeshita",
year = "2003",
month = "2",
day = "1",
doi = "10.1161/01.HYP.0000050649.30821.CB",
language = "English",
volume = "41",
pages = "255--260",
journal = "Hypertension",
issn = "0194-911X",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Overexpression of eNOS in RVLM improves impaired baroreflex control of heart rate in SHRSP

AU - Kishi, Takuya

AU - Hirooka, Yoshitaka

AU - Kimura, Yoshikuni

AU - Sakai, Koji

AU - Ito, Koji

AU - Shimokawa, Hiroaki

AU - Takeshita, Akira

PY - 2003/2/1

Y1 - 2003/2/1

N2 - We previously demonstrated that the overexpression of endothelial nitric oxide synthase (eNOS) in the rostral ventrolateral medulla (RVLM) decreases blood pressure, heart rate (HR), and sympathetic nerve activity and that these effects are enhanced in stroke-prone spontaneously hypertensive rats (SHRSP). The aim of this study was to determine if an increase in NO production in the RVLM caused by the overexpression of eNOS improves the impaired baroreflex control of HR in SHRSP. We transfected adenovirus vectors encoding eNOS (AdeNOS) into the RVLM of SHRSP or Wistar-Kyoto rats (WKY). Mean arterial pressure and HR were measured by a radio-telemetry system in the conscious state. Reflex changes in HR were elicited by intravenous infusion of either phenylephrine, sodium nitroprusside, or hydralazine at day 7 after the gene transfer. The maximum gain of the baroreflex control of HR was significantly decreased in SHRSP compared with WKY. Overexpression of eNOS in the RVLM of SHRSP improved the impaired maximum gain of the baroreflex control of HR. After treatment with atropine, the maximum gain was still significantly greater in SHRSP in the AdeNOS-transfected group than in the nontransfected group, although it was decreased in both groups. In contrast, after treatment with metoprolol, the maximum gain did not differ between the two groups. These results indicate that an increase in NO production in the RVLM improves the impaired baroreflex control of HR in SHRSP and that these effects may have resulted from a cardiac sympathoinhibitory effect of NO in the RVLM of SHRSP.

AB - We previously demonstrated that the overexpression of endothelial nitric oxide synthase (eNOS) in the rostral ventrolateral medulla (RVLM) decreases blood pressure, heart rate (HR), and sympathetic nerve activity and that these effects are enhanced in stroke-prone spontaneously hypertensive rats (SHRSP). The aim of this study was to determine if an increase in NO production in the RVLM caused by the overexpression of eNOS improves the impaired baroreflex control of HR in SHRSP. We transfected adenovirus vectors encoding eNOS (AdeNOS) into the RVLM of SHRSP or Wistar-Kyoto rats (WKY). Mean arterial pressure and HR were measured by a radio-telemetry system in the conscious state. Reflex changes in HR were elicited by intravenous infusion of either phenylephrine, sodium nitroprusside, or hydralazine at day 7 after the gene transfer. The maximum gain of the baroreflex control of HR was significantly decreased in SHRSP compared with WKY. Overexpression of eNOS in the RVLM of SHRSP improved the impaired maximum gain of the baroreflex control of HR. After treatment with atropine, the maximum gain was still significantly greater in SHRSP in the AdeNOS-transfected group than in the nontransfected group, although it was decreased in both groups. In contrast, after treatment with metoprolol, the maximum gain did not differ between the two groups. These results indicate that an increase in NO production in the RVLM improves the impaired baroreflex control of HR in SHRSP and that these effects may have resulted from a cardiac sympathoinhibitory effect of NO in the RVLM of SHRSP.

UR - http://www.scopus.com/inward/record.url?scp=0037313947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037313947&partnerID=8YFLogxK

U2 - 10.1161/01.HYP.0000050649.30821.CB

DO - 10.1161/01.HYP.0000050649.30821.CB

M3 - Article

C2 - 12574091

AN - SCOPUS:0037313947

VL - 41

SP - 255

EP - 260

JO - Hypertension

JF - Hypertension

SN - 0194-911X

IS - 2

ER -