Overexpression of Glutathione Peroxidase Prevents Left Ventricular Remodeling and Failure after Myocardial Infarction in Mice

Tetsuya Shiomi, Hiroyuki Tsutsui, Hidenori Matsusaka, Kimiyo Murakami, Shunji Hayashidani, Masaki Ikeuchi, Jing Wen, Toro Kubota, Hideo Utsumi, Akira Takeshita

Research output: Contribution to journalArticlepeer-review

182 Citations (Scopus)

Abstract

Background-Oxidative stress plays an important role in the pathophysiology of heart failure. We determined whether the overexpression of glutathione peroxidase (GSHPx) could attenuate left ventricular (LV) remodeling and failure after myocardial infarction (MI). Methods and Results-We created MI in 12- to 16-week-old, male GSHPx transgenic mice (TG+MI) and nontransgenic wild-type littermates (WT+MI) by ligating the left coronary artery. GSHPx activity was increased in the hearts of TG mice, with no significant changes in other antioxidant enzymes. LV concentrations of thiobarbituric acid-reactive substances measured in TG+MI at 4 weeks were significantly lower than those in WT+MI. The survival rate during 4 weeks of MI was significantly higher in TG+MI than in WT+MI, although the infarct size was comparable. LV cavity dilatation and dysfunction were significantly attenuated in TG+MI. LV end-diastolic pressure was increased in WT+MI and reduced in TG+MI. Improvement of LV function in TG+MI was accompanied by a decrease in myocyte hypertrophy, apoptosis, and interstitial fibrosis in the noninfarcted LV. Myocardial matrix metalloproteinase-9 zymographic and protein levels were increased in WT+MI after 3 days but were attenuated in TG+MI. Conclusions-Overexpression of GSHPx inhibited LV remodeling and failure after MI. Therapies designed to interfere with oxidative stress might be beneficial to prevent cardiac failure.

Original languageEnglish
Pages (from-to)544-549
Number of pages6
JournalCirculation
Volume109
Issue number4
DOIs
Publication statusPublished - Feb 3 2004

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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