Overexpression of human acyl-CoA thioesterase upregulates peroxisome biogenesis

Mitsuru Ishizuka, Yoshiro Toyama, Hiroyuki Watanabe, Yukio Fujiki, Arata Takeuchi, Shou Yamasaki, Shigeki Yuasa, Masaru Miyazaki, Nobuyuki Nakajima, Shinsuke Taki, Takashi Saito

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    The biological functions of human acyl-CoA thioesterase III (ACTEIII/PTE-1), initially identified as an HIV-1 Nef binding protein, have remained unclear. We report herein that the stable overexpression of ACTEIII/PTE-1 in human and murine T-cell lines resulted in an increase in both peroxisome number and lipid droplet formation in a manner dependent on the amount of the protein. Peroxisome proliferation was evidenced by immunofluorescence staining for catalase, a peroxisome marker protein, as well as by direct peroxisome enumeration on electron micrographs. Consistently, the amount of catalase was elevated as the amount of ACTEIII/PTE-1 was increased. ACTEIII/PTE-1 mutants with reduced enzymatic activity or with the defect in peroxisome localization did not induce peroxisome proliferation, indicating that peroxisome proliferation was mediated by metabolites generated by ACTEIII/PTE-1 within peroxisomes. Finally, thymocytes isolated from a T-cell-specific ACTEIII/PTE-1 transgenic mouse as well as human and murine cell lines of lymphoid and non-lymphoid origins exhibited a similar proliferation of peroxisomes. Thus, ACTEIII/PTE-1 may be involved in the metabolic regulation of peroxisome proliferation.

    Original languageEnglish
    Pages (from-to)127-141
    Number of pages15
    JournalExperimental Cell Research
    Volume297
    Issue number1
    DOIs
    Publication statusPublished - Jul 1 2004

    Fingerprint

    Acyl Coenzyme A
    Peroxisomes
    Up-Regulation
    Catalase
    T-Lymphocytes
    Cell Line
    Thymocytes
    Transgenic Mice
    Fluorescent Antibody Technique
    Carrier Proteins
    Proteins
    Electrons
    Staining and Labeling

    All Science Journal Classification (ASJC) codes

    • Cell Biology

    Cite this

    Ishizuka, M., Toyama, Y., Watanabe, H., Fujiki, Y., Takeuchi, A., Yamasaki, S., ... Saito, T. (2004). Overexpression of human acyl-CoA thioesterase upregulates peroxisome biogenesis. Experimental Cell Research, 297(1), 127-141. https://doi.org/10.1016/j.yexcr.2004.02.029

    Overexpression of human acyl-CoA thioesterase upregulates peroxisome biogenesis. / Ishizuka, Mitsuru; Toyama, Yoshiro; Watanabe, Hiroyuki; Fujiki, Yukio; Takeuchi, Arata; Yamasaki, Shou; Yuasa, Shigeki; Miyazaki, Masaru; Nakajima, Nobuyuki; Taki, Shinsuke; Saito, Takashi.

    In: Experimental Cell Research, Vol. 297, No. 1, 01.07.2004, p. 127-141.

    Research output: Contribution to journalArticle

    Ishizuka, M, Toyama, Y, Watanabe, H, Fujiki, Y, Takeuchi, A, Yamasaki, S, Yuasa, S, Miyazaki, M, Nakajima, N, Taki, S & Saito, T 2004, 'Overexpression of human acyl-CoA thioesterase upregulates peroxisome biogenesis', Experimental Cell Research, vol. 297, no. 1, pp. 127-141. https://doi.org/10.1016/j.yexcr.2004.02.029
    Ishizuka M, Toyama Y, Watanabe H, Fujiki Y, Takeuchi A, Yamasaki S et al. Overexpression of human acyl-CoA thioesterase upregulates peroxisome biogenesis. Experimental Cell Research. 2004 Jul 1;297(1):127-141. https://doi.org/10.1016/j.yexcr.2004.02.029
    Ishizuka, Mitsuru ; Toyama, Yoshiro ; Watanabe, Hiroyuki ; Fujiki, Yukio ; Takeuchi, Arata ; Yamasaki, Shou ; Yuasa, Shigeki ; Miyazaki, Masaru ; Nakajima, Nobuyuki ; Taki, Shinsuke ; Saito, Takashi. / Overexpression of human acyl-CoA thioesterase upregulates peroxisome biogenesis. In: Experimental Cell Research. 2004 ; Vol. 297, No. 1. pp. 127-141.
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