Overexpression of ubiquitin carboxyl-terminal hydrolase L1 arrests spermatogenesis in transgenic mice

Yu Lai Wang, Wanzhao Liu, Ying Jie Sun, Jungkee Kwon, Rieko Setsuie, Hitoshi Osaka, Mami Noda, Shunsuke Aoki, Yasuhiro Yoshikawa, Keiji Wada

Research output: Contribution to journalArticle

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Abstract

Ubiquitin carboxyl-terminal hydrolase 1 (UCH-L1) can be detected in mouse testicular germ cells, mainly spermatogonia and somatic Sertoli cells, but its physiological role is unknown. We show that transgenic (Tg) mice overexpressing EF1α promoter-driven UCH-L1 in the testis are sterile due to a block during spermatogenesis at an early stage (pachytene) of meiosis. interestingly, almost all spermatogonia and Sertoli cells expressing excess UCH-L1, but little PCNA (proliferating cell nuclear antigen), showed no morphological signs of apoptosis or TUNEL-positive staining. Rather, germ cell apoptosis was mainly detected in primary spermatocytes having weak or negative UCH-L1 expression but strong PCNA expression. These data suggest that overexpression of UCH-L1 affects spermatogenesis during meiosis and, in particular, induces apoptosis in primary spermatocytes. In addition to results of caspases-3 upregulation and Bcl-2 downregulation, excess UCH-L1 influenced the distribution of PCNA, suggesting a specific role for UCH-L1 in the processes of mitotic proliferation and differentiation of spermatogonial stem cells during spermatogenesis.

Original languageEnglish
Pages (from-to)40-49
Number of pages10
JournalMolecular Reproduction and Development
Volume73
Issue number1
DOIs
Publication statusPublished - Jan 1 2006

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Hydrolases
Ubiquitin
Transgenic Mice
Proliferating Cell Nuclear Antigen
Spermatogenesis
Spermatogonia
Spermatocytes
Sertoli Cells
Meiosis
Apoptosis
Germ Cells
Pachytene Stage
In Situ Nick-End Labeling
Arrest of spermatogenesis
Caspase 3
Testis
Up-Regulation
Stem Cells
Down-Regulation
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Overexpression of ubiquitin carboxyl-terminal hydrolase L1 arrests spermatogenesis in transgenic mice. / Wang, Yu Lai; Liu, Wanzhao; Sun, Ying Jie; Kwon, Jungkee; Setsuie, Rieko; Osaka, Hitoshi; Noda, Mami; Aoki, Shunsuke; Yoshikawa, Yasuhiro; Wada, Keiji.

In: Molecular Reproduction and Development, Vol. 73, No. 1, 01.01.2006, p. 40-49.

Research output: Contribution to journalArticle

Wang, YL, Liu, W, Sun, YJ, Kwon, J, Setsuie, R, Osaka, H, Noda, M, Aoki, S, Yoshikawa, Y & Wada, K 2006, 'Overexpression of ubiquitin carboxyl-terminal hydrolase L1 arrests spermatogenesis in transgenic mice', Molecular Reproduction and Development, vol. 73, no. 1, pp. 40-49. https://doi.org/10.1002/mrd.20364
Wang, Yu Lai ; Liu, Wanzhao ; Sun, Ying Jie ; Kwon, Jungkee ; Setsuie, Rieko ; Osaka, Hitoshi ; Noda, Mami ; Aoki, Shunsuke ; Yoshikawa, Yasuhiro ; Wada, Keiji. / Overexpression of ubiquitin carboxyl-terminal hydrolase L1 arrests spermatogenesis in transgenic mice. In: Molecular Reproduction and Development. 2006 ; Vol. 73, No. 1. pp. 40-49.
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