Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus

Hirokazu Nishida, Daisuke Tsuchiya, Yoshizumi Ishino, Kosuke Morikawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

DNA ligases seal single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome during various aspects of DNA metabolism, such as replication, excision repair and recombination. DNA-strand breaks are frequently generated as reaction intermediates in these events and the sealing of these breaks depends solely on the proper function of DNA ligase. Crystals of the archaeal DNA ligase from Pyrococcus furiosus were obtained using 6.6%(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 Å resolution. They belong to the monoclinic space group P21, with unit-cell parameters a = 61.1, b = 88.3, c = 63.4 Å, β = 108.9°. The asymmetric unit contains one ligase molecule.

Original languageEnglish
Pages (from-to)1100-1102
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number12
DOIs
Publication statusPublished - Dec 1 2005

Fingerprint

Archaeal DNA
Pyrococcus furiosus
DNA Ligases
Crystallization
purification
Purification
deoxyribonucleic acid
crystallization
DNA
Reaction intermediates
DNA Breaks
Double-Stranded DNA Breaks
Ligases
Metabolism
DNA Repair
strands
Genetic Recombination
Seals
Repair
Ethanol

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus. / Nishida, Hirokazu; Tsuchiya, Daisuke; Ishino, Yoshizumi; Morikawa, Kosuke.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 12, 01.12.2005, p. 1100-1102.

Research output: Contribution to journalArticle

@article{5382c0d1cb654c44986b5041f722e31c,
title = "Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus",
abstract = "DNA ligases seal single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome during various aspects of DNA metabolism, such as replication, excision repair and recombination. DNA-strand breaks are frequently generated as reaction intermediates in these events and the sealing of these breaks depends solely on the proper function of DNA ligase. Crystals of the archaeal DNA ligase from Pyrococcus furiosus were obtained using 6.6{\%}(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 {\AA} resolution. They belong to the monoclinic space group P21, with unit-cell parameters a = 61.1, b = 88.3, c = 63.4 {\AA}, β = 108.9°. The asymmetric unit contains one ligase molecule.",
author = "Hirokazu Nishida and Daisuke Tsuchiya and Yoshizumi Ishino and Kosuke Morikawa",
year = "2005",
month = "12",
day = "1",
doi = "10.1107/S1744309105038649",
language = "English",
volume = "61",
pages = "1100--1102",
journal = "Acta Crystallographica Section F:Structural Biology Communications",
issn = "1744-3091",
publisher = "John Wiley and Sons Ltd",
number = "12",

}

TY - JOUR

T1 - Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus

AU - Nishida, Hirokazu

AU - Tsuchiya, Daisuke

AU - Ishino, Yoshizumi

AU - Morikawa, Kosuke

PY - 2005/12/1

Y1 - 2005/12/1

N2 - DNA ligases seal single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome during various aspects of DNA metabolism, such as replication, excision repair and recombination. DNA-strand breaks are frequently generated as reaction intermediates in these events and the sealing of these breaks depends solely on the proper function of DNA ligase. Crystals of the archaeal DNA ligase from Pyrococcus furiosus were obtained using 6.6%(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 Å resolution. They belong to the monoclinic space group P21, with unit-cell parameters a = 61.1, b = 88.3, c = 63.4 Å, β = 108.9°. The asymmetric unit contains one ligase molecule.

AB - DNA ligases seal single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome during various aspects of DNA metabolism, such as replication, excision repair and recombination. DNA-strand breaks are frequently generated as reaction intermediates in these events and the sealing of these breaks depends solely on the proper function of DNA ligase. Crystals of the archaeal DNA ligase from Pyrococcus furiosus were obtained using 6.6%(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 Å resolution. They belong to the monoclinic space group P21, with unit-cell parameters a = 61.1, b = 88.3, c = 63.4 Å, β = 108.9°. The asymmetric unit contains one ligase molecule.

UR - http://www.scopus.com/inward/record.url?scp=33744472228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744472228&partnerID=8YFLogxK

U2 - 10.1107/S1744309105038649

DO - 10.1107/S1744309105038649

M3 - Article

C2 - 16511246

AN - SCOPUS:33744472228

VL - 61

SP - 1100

EP - 1102

JO - Acta Crystallographica Section F:Structural Biology Communications

JF - Acta Crystallographica Section F:Structural Biology Communications

SN - 1744-3091

IS - 12

ER -