Overhauser-enhanced magnetic resonance imaging characterization of mitochondria functional changes in the 6-hydroxydopamine rat model

Mayumi Yamato, Takeshi Shiba, Tatsuya Naganuma, Kazuhiro Ichikawa, Hideo Utsumi, Ken-Ichi Yamada

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Oxidative stress may be involved in the dopaminergic neurodegenerations seen in 6-OHDA-lesioned rats through its production of free radicals and through mitochondrial dysfunction. In this study, we noninvasively demonstrate brain redox alterations in 6-OHDA-lesioned rats using Overhauser-enhanced magnetic resonance imaging (OMRI). The reduction rate of 3-methoxycarbonyl-2,2,5,5- tetramethylpyrrolidine-l-oxyl (methoxycarbonyl-PROXYL), a redox-sensitive contrast agent, was used as an index of the redox status in vivo. The methoxycarbonyl-PROXYL reduction rate, calculated from continuous images, decreased significantly in lesioned hemispheres compared to their corresponding contralateral hemispheres. The reduction rates in cellular fractions obtained from the striatum were estimated by X-band electron spin resonance (ESR) and calculated by assuming first-order kinetics for their time-dependent decreases. When methoxycarbonyl-PROXYL was mixed with cytoplasm fractions, the reduction rates were the same in both hemispheres. However, the ESR signal of methoxycarbonyl-PROXYL in the mitochondrial fraction of the lesioned hemispheres decayed more slowly than that of the corresponding contralateral hemispheres. Concordantly, biochemical assays showed that the activity of mitochondrial complex I also decreased more slowly in lesioned hemispheres. Thus, this method of noninvasively imaging brain redox alterations faithfully reflects changes in mitochondrial complex I activity in 6-OHDA-lesioned rats.

Original languageEnglish
Pages (from-to)804-811
Number of pages8
JournalNeurochemistry International
Volume59
Issue number6
DOIs
Publication statusPublished - Nov 1 2011

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Oxidopamine
Oxidation-Reduction
Mitochondria
Magnetic Resonance Imaging
Electron Spin Resonance Spectroscopy
Neuroimaging
Contrast Media
Free Radicals
Cytoplasm
Oxidative Stress
Brain

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Cell Biology

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Overhauser-enhanced magnetic resonance imaging characterization of mitochondria functional changes in the 6-hydroxydopamine rat model. / Yamato, Mayumi; Shiba, Takeshi; Naganuma, Tatsuya; Ichikawa, Kazuhiro; Utsumi, Hideo; Yamada, Ken-Ichi.

In: Neurochemistry International, Vol. 59, No. 6, 01.11.2011, p. 804-811.

Research output: Contribution to journalArticle

Yamato, Mayumi ; Shiba, Takeshi ; Naganuma, Tatsuya ; Ichikawa, Kazuhiro ; Utsumi, Hideo ; Yamada, Ken-Ichi. / Overhauser-enhanced magnetic resonance imaging characterization of mitochondria functional changes in the 6-hydroxydopamine rat model. In: Neurochemistry International. 2011 ; Vol. 59, No. 6. pp. 804-811.
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