Overview: Engineering transgenic constructs and mice

Naoto Haruyama, Andrew Cho, Ashok B. Kulkarni

Research output: Contribution to journalReview article

39 Citations (Scopus)

Abstract

Cell biology research encompasses everything from single cells to whole animals. Recent discoveries concerning particular gene functions can be applied to the whole animal for understanding genotype-phenotype relationships underlying disease mechanisms. For this reason, genetically manipulated mousemodels are nowconsidered essential to correctly understand disease processes in whole animals. This unit reviews the basic mouse technologies used to generate conventional transgenic mice, which represent a gain-of-function approach. First, an overview of transgenic construct design is presented. This unit then explains basic strategies for the identification and establishment of independent transgenic mouse lines, followed by comments on historical and emerging techniques. It then describes typical problems that are encountered when researchers start to generate transgenic mice.

Original languageEnglish
JournalCurrent Protocols in Cell Biology
Issue numberSUPPL. 42
DOIs
Publication statusPublished - Apr 8 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

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