Oxidative damage and brain concentrations of free amino acid in chicks exposed to high ambient temperature

Vishwajit S. Chowdhury, Shozo Tomonaga, Taro Ikegami, Edi Erwan, Kentaro Ito, John F. Cockrem, Mitsuhiro Furuse

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

High ambient temperatures (HT) reduce food intake and body weight in young chickens, and HT can cause increased expression of hypothalamic neuropeptides. The mechanisms by which HT act, and the effects of HT on cellular homeostasis in the brain, are however not well understood. In the current study lipid peroxidation and amino acid metabolism were measured in the brains of 14 d old chicks exposed to HT (35. °C for 24- or 48-h) or to control thermoneutral temperature (CT; 30. °C). Malondialdehyde (MDA) was measured in the brain to determine the degree of oxidative damage. HT increased body temperature and reduced food intake and body weight gain. HT also increased diencephalic oxidative damage after 48. h, and altered some free amino acid concentrations in the diencephalon. Diencephalic MDA concentrations were increased by HT and time, with the effect of HT more prominent with increasing time. HT altered cystathionine, serine, tyrosine and isoleucine concentrations. Cystathionine was lower in HT birds compared with CT birds at 24. h, whilst serine, tyrosine and isoleucine were higher at 48. h in HT birds. An increase in oxidative damage and alterations in amino acid concentrations in the diencephalon may contribute to the physiological, behavioral and thermoregulatory responses of heat-exposed chicks.

Original languageEnglish
Pages (from-to)70-76
Number of pages7
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume169
DOIs
Publication statusPublished - Mar 2014

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

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