Oxidative stress impairs insulin signal in skeletal muscle and causes insulin resistance in postinfarct heart failure

Yukihiro Ohta, Shintaro Kinugawa, Shoji Matsushima, Taisuke Ono, Mochamad A. Sobirin, Naoki Inoue, Takashi Yokota, Kagami Hirabayashi, Hiroyuki Tsutsui

Research output: Contribution to journalArticle

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Abstract

Insulin resistance has been shown to occur as a consequence of heart failure. However, its exact mechanisms in this setting remain unknown. We have previously reported that oxidative stress is enhanced in the skeletal muscle from mice with heart failure after myocardial infarction (MI) (30). This study is aimed to investigate whether insulin resistance in postinfarct heart failure is due to the impairment of insulin signaling in the skeletal muscle caused by oxidative stress. Mice were divided into four groups: sham operated (sham); sham treated with apocynin, an inhibitor of NAD(P)H oxidase activation (10 mmol/l in drinking water); MI; and MI treated with apocynin. After 4 wk, intraperitoneal insulin tolerance tests were performed, and skeletal muscle samples were obtained for insulin signaling measurements. MI mice showed left ventricular dilation and dysfunction by echocardiography and increased left ventricular end-diastolic pressure and lung weight. The decrease in glucose level after insulin load significantly attenuated in MI compared with sham. Insulin-stimulated serine phosphorylation of Akt and glucose transporter-4 translocation were decreased in MI mice by 61 and 23%, respectively. Apocynin ameliorated the increase in oxidative stress and NAD(P)H oxidase activities measured by the lucigenin assay in the skeletal muscle after MI. It also improved insulin resistance and inhibited the decrease of Akt phosphorylation and glucose transporter-4 translocation. Insulin resistance was induced by the direct impairment of insulin signaling in the skeletal muscle from postinfarct heart failure, which was associated with the enhanced oxidative stress via NAD(P)H oxidase.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume300
Issue number5
DOIs
Publication statusPublished - May 1 2011
Externally publishedYes

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Insulin Resistance
Skeletal Muscle
Oxidative Stress
Heart Failure
Myocardial Infarction
Insulin
NADPH Oxidase
Facilitative Glucose Transport Proteins
Phosphorylation
Left Ventricular Dysfunction
Drinking Water
Serine
Echocardiography
Dilatation
Blood Pressure
Weights and Measures
Glucose
Lung
acetovanillone

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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Oxidative stress impairs insulin signal in skeletal muscle and causes insulin resistance in postinfarct heart failure. / Ohta, Yukihiro; Kinugawa, Shintaro; Matsushima, Shoji; Ono, Taisuke; Sobirin, Mochamad A.; Inoue, Naoki; Yokota, Takashi; Hirabayashi, Kagami; Tsutsui, Hiroyuki.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 300, No. 5, 01.05.2011.

Research output: Contribution to journalArticle

Ohta, Yukihiro ; Kinugawa, Shintaro ; Matsushima, Shoji ; Ono, Taisuke ; Sobirin, Mochamad A. ; Inoue, Naoki ; Yokota, Takashi ; Hirabayashi, Kagami ; Tsutsui, Hiroyuki. / Oxidative stress impairs insulin signal in skeletal muscle and causes insulin resistance in postinfarct heart failure. In: American Journal of Physiology - Heart and Circulatory Physiology. 2011 ; Vol. 300, No. 5.
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